Countess of Pembroke is a title that has been borne by several women throughout history, including:
- Aoife MacMurrough (c. 1145 – 1188), Irish noblewoman, Princess of Leinster
- Isabel de Clare, 4th Countess of Pembroke (1172–1220), wife of William Marshal, 1st Earl of Pembroke, and Countess of Pembroke in her own right
- Mary de St Pol, (c.1303–1377), wife of Aymer de Valence, 2nd Earl of Pembroke (c.1275–1324)
- Margaret of England (1346–1361), wife of John Hastings, 2nd Earl of Pembroke; daughter of King Edward III and Philippa of Hainault.
- Anne Manny (1355–1384), 2nd wife to John Hastings, 2nd Earl of Pembroke; daughter of Walter Manny, Baron Manny and Margaret, Duchess of Norfolk.
- Anne Devereux (c.1430–c.1486), wife of William Herbert, 1st Earl of Pembroke.
- Mary Woodville (c.1456–1481), wife of William Herbert, 2nd Earl of Pembroke. Title attained in 1479.
- Catherine Woodville (1458–1497), wife of Jasper Tudor, 1st Earl of Pembroke.
- Anne Parr (1515–1552), wife of William Herbert, 1st Earl of Pembroke and sister of Queen consort Catherine Parr.
- Mary Sidney (1561–1621), wife of Henry Herbert, 2nd Earl of Pembroke; one of the first English women to achieve a major reputation for her literary works, translations and literary patronage.
- Mary Talbot (circa 1594–1649), wife of William Herbert, 3rd Earl of Pembroke.
- Elizabeth Spencer (1737–1831), wife of Henry Herbert, 10th Earl of Pembroke daughter of Charles Spencer, 3rd Duke of Marlborough and Elizabeth Trevor.