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Izayoi (written: 十六夜 lit. "Sixteenth Night"), is a Japanese family surname. Fictional people with the surname include:

• Izayoi, a character from the Japanese manga series Inuyasha.
• Izayoi, a character from the BlazBlue fighting game series.
• Sakamaki Izayoi, the main protagonist from the Japanese light novel series Problem Children are Coming from Another World, aren't they?.
• Sakuya Izayoi, a character from bullet hell shoot 'em up video game series Touhou Project.
• Miku Izayoi, a character from the a Japanese light novel series Date A Live.
• Aki Izayoi, a character from the second main spin-off of the Yu-Gi-Oh! franchise, Yu-Gi-Oh! 5D's.
• Kyuemon Izayoi, also known as Kyuemon Shingetsu Kibaoni, a secondary villain from the 39th season of Super Sentai Series, Shuriken Sentai Ninninger.
• Sonosuke Izayoi, a character from the second anime series based in the Danganronpa video game, Danganronpa 3: The End of Hope's Peak Academy.

• Disambiguation pages
• Japanese-language surnames