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Leptospira alstonii
Scientific classification
Domain:	Bacteria
Phylum:	Spirochaetota
Class:	Spirochaetia
Order:	Leptospirales
Family:	Leptospiraceae
Genus:	Leptospira
Species:	L. alstonii
Binomial name
Leptospira alstonii Smythe et al. 2013

Leptospira alstonii is a gram negative, mobile, spirochete. It is flexible, helical, and motile by means of two periplasmic flagella (axial fibrils). It is obligately aerobic and oxidase positive. It was named after J. M. Alston, a British microbiologist who made significant contributions to the study of Leptospirosis. It is one of nine human or animal pathogenic species of Leptospira. It was originally isolated from material submitted to the Veterinary Diagnostic Laboratory at Iowa State University during an outbreak of swine abortion in 1983. It has been isolated and stored in liquid nitrogen or Ellinghausen-McCullough-Johnson-Harris medium. It also has been isolated in China from a frog. The strain is also available from culture collections of the WHO collaborating centers. Lipase is not produced by this species. NaCl is not required for growth. Growth is inhibited by 8-azaguanine at 225 μg/mL or 2,6-diaminopurine (10 μg/mL) and copper sulfate. It contains serovars from the serogroup ranarum. DNA G+C content is 39±8 mol%.

References

1. Haake, D A; Champion, C I; Martinich, C; Shang, E S; Blanco, D R; Miller, J N; Lovett, M A (July 1993). "Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp". Journal of Bacteriology. 175 (13): 4225–4234. doi:10.1128/jb.175.13.4225-4234.1993. PMC 204853. PMID 8320237.
2. ^ Smythe, L.; Adler, B.; Hartskeerl, R. A.; Galloway, R. L.; Turenne, C. Y.; Levett, P. N. (14 September 2012). "Classification of Leptospira genomospecies 1, 3, 4 and 5 as Leptospira alstonii sp. nov., Leptospira vanthielii sp. nov., Leptospira terpstrae sp. nov. and Leptospira yanagawae sp. nov., respectively". International Journal of Systematic and Evolutionary Microbiology. 63 (Pt 5): 1859–1862. doi:10.1099/ijs.0.047324-0. PMID 22984140.
3. Meny, Paulina; Menéndez, Clara; Quintero, Jair; Hernández, Elba; Ríos, Cristina; Balassiano, Ilana Teruszkin; Trindade, Camilla Nunes Dos Reis; Vital-Brazil, Juliana Magalhães; Ramos, Tatiane Mendes Varela; Ashfield, Natalia; Feble, Camila; Avila, Esthefani; Schelotto, Felipe; Varela, Gustavo (21 December 2017). "Characterization of Leptospira isolates from humans and the environment in Uruguay". Revista do Instituto de Medicina Tropical de São Paulo. 59: e79. doi:10.1590/s1678-9946201759079. PMC 5738764. PMID 29267587.
4. Miller, David A.; Wilson, Mark A.; Owen, William J.; Beran, George W. (25 June 2016). "Porcine Leptospirosis in Iowa". Journal of Veterinary Diagnostic Investigation. 2 (3): 171–175. doi:10.1177/104063879000200303. PMID 2094443.
5. Alexander, A. D., E. F. Lessel, L. B. Evans, E. Franck, and S. S. Green. 1972. Preservation of leptospiras by liquid-nitrogen refrigeration. Int. J. Syst. Bacteriol. 22:165-169.

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