The Mississippi Valley is a valley which the Mississippi River flows through.
Mississippi Valley may also refer to:
- Mississippi Valley Airlines, former regional airline in the Upper Midwest of the United States
- Mississippi Valley Conference (disambiguation)
- Mississippi Valley Division, military unit responsible for the area surrounding the Mississippi River
- Mississippi Valley League, former baseball Class D minor league
- Mississippi Valley State University, public HBCU located in Itta Bena, Mississippi
- Mississippi-Valley type (MVT), a type of Sphalerite
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