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In enzymology, a nicotine dehydrogenase (EC 1.5.99.4) is an enzyme that catalyzes the chemical reaction

(S)-nicotine + acceptor + H₂O ${\displaystyle \rightleftharpoons }$ (S)-6-hydroxynicotine + reduced acceptor

The 3 substrates of this enzyme are (S)-nicotine, acceptor, and H₂O, whereas its two products are (S)-6-hydroxynicotine and reduced acceptor.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is nicotine:acceptor 6-oxidoreductase (hydroxylating). Other names in common use include nicotine oxidase, D-nicotine oxidase, nicotine:(acceptor) 6-oxidoreductase (hydroxylating), and L-nicotine oxidase. It has 2 cofactors: metal, and FMN.

References

• BEHRMAN EJ, STANIER RY (1957). "The bacterial oxidation of nicotinic acid". J. Biol. Chem. 228 (2): 923–45. doi:10.1016/S0021-9258(18)70671-6. PMID 13475371.
• Decker K, Bleeg H (1965). "Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans". Biochim. Biophys. Acta. 105 (2): 313–24. doi:10.1016/s0926-6593(65)80155-2. PMID 5849820.
• Hochstein LI, Dalton BP (1967). "The purification and properties of nicotine oxidase". Biochim. Biophys. Acta. 139 (1): 56–68. doi:10.1016/0005-2744(67)90113-1. PMID 4962139.
• HOCHSTEIN LI, RITTENBERG SC (1959). "The bacterial oxidation of nicotine. II. The isolation of the first oxidative product and its identification as (1)-6-hydroxynicotine". J. Biol. Chem. 234 (1): 156–60. doi:10.1016/S0021-9258(18)70355-4. PMID 13610912.

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