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Phage immunoprecipitation sequencing (PhIP-Seq) is method that combines barcoded DNA high-throughput sequencing and proteomics to determine the levels of binding of antibodies to epitopes. It has been used to study the autoantibody repertoire of autoimmune diseases like multiple sclerosis, type 2 diabetes and rheumatoid arthritis.
References
- Larman, H; et al. (2011). "Autoantigen discovery with a synthetic human peptidome". Nature Biotechnology. 29 (6): 535–541. doi:10.1038/nbt.1856. PMC 4169279. PMID 21602805.
- Larman, H; et al. (June 2013). "PhIP-Seq characterization of autoantibodies from patients with multiple sclerosis, type 1 diabetes and rheumatoid arthritis". Journal of Autoimmunity. 43: 1–9. doi:10.1016/j.jaut.2013.01.013. PMC 3677742. PMID 23497938.
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