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{{Short description|Chemical solution used in RNA/DNA/protein extraction}}
'''Trizol''' is the brand name of a solution used in RNA/DNA/protein extraction from Invitrogen<ref></ref> and is generally used to describe the method even when using another company's solution. The correct name of the method is ]. Trizol can give good yield for DNA and RNA. An alternative method for ] is phenol extraction and TCA/acetone precipitation.
] and ].]]


'''TRIzol''' is a widely used<ref>{{Cite web|title=Total RNA isolation: Trizol vs RNeasy|url=http://www.bioastrum.com/home/content/total-rna-isolation-trizol-vs-rneasy|access-date=2021-08-18|website=www.bioastrum.com|language=en}}</ref> chemical solution used in the extraction of ], ], and ] from cells. The solution was initially used and published by ] and ] in 1987.<ref>{{cite journal| pmid=2440339 | doi=10.1016/0003-2697(87)90021-2 | volume=162 | title=Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction | date=April 1987 | journal=Anal. Biochem. | pages=156–9 | last1 = Chomczynski | first1 = P | last2 = Sacchi | first2 = N| issue=1 }}</ref>
⚫ TRIZOL is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.


TRIzol is the brand name of ] from the Ambion part of ],<ref></ref> and Tri-Reagent is the brand name from MRC,<ref></ref> which was founded by Chomczynski.
⚫ When used, it looks exactly as if it were cough syrup, bright pink. The smell, more specifically, the smell of the ] and guanidinium thiocyanate, are extremely strong. TRIZOL works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.


==Uses in extraction==
It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIZOL (specifically ]) and Chloroform.
The correct name of the method is ]. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield.<ref>{{Cite web |title=RNA isolation |url=https://depts.washington.edu/flowlab/Cell%20Analysis%20Facility/Cell%20sorting%20for%20RNA%20isolation.pdf |url-status=dead |website=Cell Analysis Facility |access-date=2021-08-18 |archive-date=2021-08-18 |archive-url=https://web.archive.org/web/20210818142832/https://depts.washington.edu/flowlab/Cell%20Analysis%20Facility/Cell%20sorting%20for%20RNA%20isolation.pdf }}</ref><ref>{{Cite web|title=Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database|url=https://brd.nci.nih.gov/brd/paper/cardiovasc-pathol/2015/evaluation-of-optimal-rna-extraction-method-from-human-carotid/123390#:~:text=RNA%20yields%20were%20approximately%204.5-fold%20higher%20from%20snap-frozen,numbers%20(RIN)%20were%20significantly%20lower%20than%20using%20RNEasy.|access-date=2021-08-18|website=brd.nci.nih.gov}}</ref> An alternative method for ] is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.

DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct-zol Miniprep kit by ].<ref>{{Cite web|url=https://www.zymoresearch.com/collections/direct-zol-rna-kits/products/direct-zol-rna-microprep-kits|title=Direct-zol RNA Microprep Kits}}</ref> This method eliminates the use of Chloroform and 1-bromo-3-chloropropane completely, bypassing phase-separation and precipitation steps.<ref>{{Cite web|url=https://patents.google.com/patent/EP2770056A1|title=Nucleic acid purification}}</ref>

⚫ TRIzol is light-sensitive and is often stored in a dark-colored, glass container covered in foil. It is stored at room temperature.

⚫ When used, it resembles cough syrup, bright pink. The smell of the ] is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.


== Hazards == == Hazards ==
Vigilant caution should be taken while using TRIzol (due to the ] and ]).
Exposure to Trizol can be a serious health hazard. While mild exposure to skin can cause irritation and loss of sensation, overexposure can lead to respiratory arrest, seizure and death<ref></ref>

TRIzol is labeled as acute oral, dermal, and inhalation toxicity besides skin corrosion/irritation in the manufacturer MDS.<ref>{{Cite web|title=TRIZOl MDS|url=http://tools.thermofisher.com/content/sfs/msds/2012/15596026_MTR-NALT_EN.pdf}}</ref>

Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns, permanent scarring and kidney failure.

Experiments should be performed under a chemical hood, with lab coat, nitrile gloves and a plastic apron. <ref> {{Webarchive|url=https://web.archive.org/web/20080721185232/http://cellbiology.med.unsw.edu.au/units/lab/msds/Trizol.pdf |date=2008-07-21 }}</ref><ref> {{Webarchive|url=https://web.archive.org/web/20081228194341/http://mrcgene.com/msdsrna.htm |date=2008-12-28 }}</ref>

TRIzol waste should never be mixed with bleach or acids: the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases.


== References == == References ==
{{Reflist}} {{Reflist}}

== External links ==
*


] ]
] ]

{{product-stub}}

Latest revision as of 23:09, 12 November 2024

Chemical solution used in RNA/DNA/protein extraction
TRIzol reagent contains guanidinium thiocyanate and phenol.

TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.

TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies, and Tri-Reagent is the brand name from MRC, which was founded by Chomczynski.

Uses in extraction

The correct name of the method is guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield. An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.

DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct-zol Miniprep kit by Zymo Research. This method eliminates the use of Chloroform and 1-bromo-3-chloropropane completely, bypassing phase-separation and precipitation steps.

TRIzol is light-sensitive and is often stored in a dark-colored, glass container covered in foil. It is stored at room temperature.

When used, it resembles cough syrup, bright pink. The smell of the phenol is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.

Hazards

Vigilant caution should be taken while using TRIzol (due to the phenol and chloroform).

TRIzol is labeled as acute oral, dermal, and inhalation toxicity besides skin corrosion/irritation in the manufacturer MDS.

Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns, permanent scarring and kidney failure.

Experiments should be performed under a chemical hood, with lab coat, nitrile gloves and a plastic apron.

TRIzol waste should never be mixed with bleach or acids: the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases.

References

  1. "Total RNA isolation: Trizol vs RNeasy". www.bioastrum.com. Retrieved 2021-08-18.
  2. Chomczynski, P; Sacchi, N (April 1987). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Anal. Biochem. 162 (1): 156–9. doi:10.1016/0003-2697(87)90021-2. PMID 2440339.
  3. Life Technologies
  4. Molecular Research Center, Inc.
  5. "RNA isolation" (PDF). Cell Analysis Facility. Archived from the original (PDF) on 2021-08-18. Retrieved 2021-08-18.
  6. "Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database". brd.nci.nih.gov. Retrieved 2021-08-18.
  7. "Direct-zol RNA Microprep Kits".
  8. "Nucleic acid purification".
  9. "TRIZOl MDS" (PDF).
  10. Material Safety data sheet from Life technologies. Archived 2008-07-21 at the Wayback Machine
  11. Material Safety data sheet from MRC Archived 2008-12-28 at the Wayback Machine

External links

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