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'''TRIzol''' is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from Chomczynski, P. and Sacchi, N. in 1987.<ref></ref> | ||
| '''Trizol''' is the brand name from ]<ref></ref> and the brand name from Chomczynski's company '''MRC'''<ref> </ref>is '''TRI Reagent'''. | |||
| The correct name of the method is ]. Trizol can give good yield for DNA and RNA. An alternative method for ] is phenol extraction and TCA/acetone precipitation. | |||
| TRIZOL is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature. | TRIZOL is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature. | ||
Revision as of 08:49, 15 December 2008
TRIzol is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from Chomczynski, P. and Sacchi, N. in 1987.
Trizol is the brand name from Invitrogen and the brand name from Chomczynski's company MRCis TRI Reagent.
The correct name of the method is Guanidinium thiocyanate-phenol-chloroform extraction. Trizol can give good yield for DNA and RNA. An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation.
TRIZOL is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.
When used, it looks exactly as if it were cough syrup, bright pink. The smell, more specifically, the smell of the phenol and guanidinium thiocyanate, are extremely strong. TRIZOL works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIZOL (specifically phenol) and Chloroform.
Hazards
Exposure to Trizol can be a serious health hazard. While mild exposure to skin can cause irritation and loss of sensation, overexposure can lead to respiratory arrest, seizure and death
References
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