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| ELISA stands for Enzyme Linked Immuno-Sorbent Assay. Essentially, this assay tells you how much protein is in any given sample. The steps in this procedure are: | |||
| 1) Attach an antibody (this antibody specifically binds to the protein of interest) to a solid support (usually a membrane) | |||
| 2) Apply the sample to this membrane, and let the protein bind to the antibody | |||
| 3) Wash the membrane | |||
| 4) Apply a second antibody which is linked to an enzyme | |||
| 5) Apply a chemical which is coverted by the enzyme into a glowing signal | |||
| 6) Count the signal | |||
| Any basic immunology or molecular biology textbook should have more information. | |||
| Try http://www.ncbi.nlm.nih.gov. They got books. | |||
Revision as of 14:54, 29 September 2002
ELISA stands for Enzyme Linked Immuno-Sorbent Assay. Essentially, this assay tells you how much protein is in any given sample. The steps in this procedure are: 1) Attach an antibody (this antibody specifically binds to the protein of interest) to a solid support (usually a membrane) 2) Apply the sample to this membrane, and let the protein bind to the antibody 3) Wash the membrane 4) Apply a second antibody which is linked to an enzyme 5) Apply a chemical which is coverted by the enzyme into a glowing signal 6) Count the signal
Any basic immunology or molecular biology textbook should have more information. Try http://www.ncbi.nlm.nih.gov. They got books.