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| ELISA stands for Enzyme Linked Immuno-Sorbent Assay. Essentially, this assay tells you if there is a certain protein in any given sample. The steps in this procedure are: | ELISA stands for ] Linked Immuno-Sorbent Assay. Essentially, this assay tells you if there is a certain ] in any given sample. The steps in this procedure are: | ||
| 1) Attach an antibody (this antibody specifically binds to the protein of interest) to a solid support (usually a membrane) | 1) Attach an ] (this ] specifically binds to the ] of interest) to a solid support (usually a membrane) | ||
| 2) Apply the sample to this membrane, and let the protein bind to the antibody | 2) Apply the sample to this membrane, and let the ] bind to the ] | ||
| 3) Wash the membrane | 3) Wash the membrane | ||
| 4) Apply a second antibody which is linked to an enzyme | 4) Apply a second ] which is linked to an ] | ||
| 5) Apply a chemical which is coverted by the enzyme |
5) Apply a chemical which is coverted by the ]into a flourescent signal | ||
| 6) See the result | 6) See the result | ||
| Any basic immunology or molecular biology textbook should have more information. | Any basic ] or ] textbook should have more information. | ||
| Try http://www.ncbi.nlm.nih.gov. They got books. | Try http://www.ncbi.nlm.nih.gov. They got books. | ||
Revision as of 18:33, 29 September 2002
ELISA stands for Enzyme Linked Immuno-Sorbent Assay. Essentially, this assay tells you if there is a certain protein in any given sample. The steps in this procedure are: 1) Attach an antibody (this antibody specifically binds to the protein of interest) to a solid support (usually a membrane) 2) Apply the sample to this membrane, and let the protein bind to the antibody 3) Wash the membrane 4) Apply a second antibody which is linked to an enzyme 5) Apply a chemical which is coverted by the enzymeinto a flourescent signal 6) See the result
Any basic immunology or molecular biology textbook should have more information. Try http://www.ncbi.nlm.nih.gov. They got books.