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ELISA stands for ] Linked Immuno-Sorbent Assay. Essentially, this assay tells you if there is a certain ] in any given sample. The steps in this procedure are: The '''Enzyme Linked Immuno-Sorbent Assay''' or '''ELISA''' is a method commonly employed in ] to detect if a certain ] is present in a given sample. The steps in this procedure are:

⚫ 1) Attach an ] (this ] specifically binds to the ] of interest) to a solid support (usually a membrane)
2) Apply the sample to this membrane, and let the ] bind to the ] # Create an ] which specifically binds to the protein of interest.
⚫ # Attach this antibody to a solid support (usually a membrane) .
3) Wash the membrane
# Apply the sample to this membrane, and let the protein bind to the antibody.
⚫ 4) Apply a second ] which is linked to an ]
# Wash the membrane, so that only the proteine bound to the antibody remains.
⚫ 5) Apply a chemical which is coverted by the ] into a flourescent signal
⚫ # Apply a second antibody which is linked to an ] and binds to the proteine of interest.
⚫ 6) See the result
⚫ # Apply a chemical which is coverted by the enzyme into a flourescent signal.
⚫ # View the result


Any basic ] or ] textbook should have more information. Any basic ] or ] textbook should have more information.

Revision as of 02:10, 30 September 2002

The Enzyme Linked Immuno-Sorbent Assay or ELISA is a method commonly employed in biochemistry to detect if a certain protein is present in a given sample. The steps in this procedure are:

  1. Create an antibody which specifically binds to the protein of interest.
  2. Attach this antibody to a solid support (usually a membrane) .
  3. Apply the sample to this membrane, and let the protein bind to the antibody.
  4. Wash the membrane, so that only the proteine bound to the antibody remains.
  5. Apply a second antibody which is linked to an enzyme and binds to the proteine of interest.
  6. Apply a chemical which is coverted by the enzyme into a flourescent signal.
  7. View the result

Any basic immunology or molecular biology textbook should have more information. Try http://www.ncbi.nlm.nih.gov. They got books.