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| # Apply the sample to this membrane, and let the protein bind to the antibody. | # Apply the sample to this membrane, and let the protein bind to the antibody. | ||
| # Wash the membrane, so that only the protein bound to the antibody remains. | # Wash the membrane, so that only the protein bound to the antibody remains. | ||
| # Apply a second antibody which is linked to an ] and binds to the |
# Apply a second antibody which is linked to an ] and binds to the protein of interest. | ||
| # Apply a chemical which is coverted by the enzyme into a flourescent signal. | # Apply a chemical which is coverted by the enzyme into a flourescent signal. | ||
| # View the result | # View the result. | ||
| Any basic ] or ] textbook should have more information. | Any basic ] or ] textbook should have more information. | ||
Revision as of 18:36, 30 September 2002
The Enzyme Linked Immuno-Sorbent Assay or ELISA is a method commonly employed in biochemistry to detect if a certain protein is present in a given sample. The steps in this procedure are:
- Create an antibody which specifically binds to the protein of interest.
- Attach this antibody to a solid support (usually a membrane) .
- Apply the sample to this membrane, and let the protein bind to the antibody.
- Wash the membrane, so that only the protein bound to the antibody remains.
- Apply a second antibody which is linked to an enzyme and binds to the protein of interest.
- Apply a chemical which is coverted by the enzyme into a flourescent signal.
- View the result.
Any basic immunology or molecular biology textbook should have more information. Try http://www.ncbi.nlm.nih.gov. They got books.