This is an old revision of this page, as edited by Evolution and evolvability (talk | contribs) at 05:52, 14 September 2019 (+ Section on "Variations" from Concluding remarks section of topicpageswiki.plos.org/Origins_of_DNA_Replication CC-BY(omitting any WP:OR comments)). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.
Revision as of 05:52, 14 September 2019 by Evolution and evolvability (talk | contribs) (+ Section on "Variations" from Concluding remarks section of topicpageswiki.plos.org/Origins_of_DNA_Replication CC-BY(omitting any WP:OR comments))(diff) ← Previous revision | Latest revision (diff) | Newer revision → (diff)The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes. This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses, such as double-stranded RNA viruses. Synthesis of daughter strands starts at discrete sites, termed replication origins, and proceeds in a bidirectional manner until all genomic DNA is replicated. Despite the fundamental nature of these events, organisms have evolved surprisingly divergent strategies that control replication onset. Although the specific replication origin organization structure and recognition varies from species to species, some common characteristics are shared.
History
In the second half of the 19th century, Gregor Mendel's pioneering work on the inheritance of traits in pea plants suggested that specific “factors” (today established as genes) are responsible for transferring organismal traits between generations. Although proteins were initially assumed to serve as the hereditary material, Avery, MacLeod and McCarty established a century later DNA, which had been discovered by Friedrich Miescher, as the carrier of genetic information. These findings paved the way for research uncovering the chemical nature of DNA and the rules for encoding genetic information, and ultimately led to the proposal of the double-helical structure of DNA by Watson and Crick. This three-dimensional model of DNA illuminated potential mechanisms by which the genetic information could be copied in a semiconservative manner prior to cell division, a hypothesis that was later experimentally supported by Meselson and Stahl using isotope incorporation to distinguish parental from newly synthesized DNA. The subsequent isolation of DNA polymerases, the enzymes that catalyze the synthesis of new DNA strands, by Kornberg and colleagues pioneered the identification of many different components of the biological DNA replication machinery, first in the bacterial model organism E. coli, but later also in eukaryotic life forms.
Features
A key prerequisite for DNA replication is that it must occur with extremely high fidelity and efficiency exactly once per cell cycle to prevent the accumulation of genetic alterations with potentially deleterious consequences for cell survival and organismal viability. Incomplete, erroneous, or untimely DNA replication events can give rise to mutations, chromosomal polyploidy or aneuploidy, and gene copy number variations, each of which in turn can lead to diseases, including cancer. To ensure complete and accurate duplication of the entire genome and the correct flow of genetic information to progeny cells, all DNA replication events are not only tightly regulated with cell cycle cues but are also coordinated with other cellular events such as transcription and DNA repair. Additionally, origin sequences commonly have high AT-content across all kingdoms, since repeats of adenine and thymine are easier to separate because their base stacking interactions are not as strong as those of guanine and cytosine.
DNA replication is divided into different stages. During initiation, the replication machineries – termed replisomes – are assembled on DNA in a bidirectional fashion. These assembly loci constitute the start sites of DNA replication or replication origins. In the elongation phase, replisomes travel in opposite directions with the replication forks, unwinding the DNA helix and synthesizing complementary daughter DNA strands using both parental strands as templates. Once replication is complete, specific termination events lead to the disassembly of replisomes. As long as the entire genome is duplicated before cell division, one might assume that the location of replication start sites does not matter; yet, it has been shown that many organisms use preferred genomic regions as origins. The necessity to regulate origin location likely arises from the need to coordinate DNA replication with other processes that act on the shared chromatin template to avoid DNA strand breaks and DNA damage.
Replicon model
More than five decades ago, Jacob, Brenner, and Cuzin proposed the replicon hypothesis to explain the regulation of chromosomal DNA synthesis in E. coli. The model postulates that a diffusible, trans-acting factor, a so-called initiator, interacts with a cis-acting DNA element, the replicator, to promote replication onset at a nearby origin. Once bound to replicators, initiators (often with the help of co-loader proteins) deposit replicative helicases onto DNA, which subsequently drive the recruitment of additional replisome components and the assembly of the entire replication machinery. The replicator thereby specifies the location of replication initiation events, and the chromosome region that is replicated from a single origin or initiation event is defined as the replicon.
A fundamental feature of the replicon hypothesis is that it relies on positive regulation to control DNA replication onset, which can explain many experimental observations in bacterial and phage systems. For example, it accounts for the failure of extrachromosomal DNAs without origins to replicate when introduced into host cells. It further rationalizes plasmid incompatibilities in E. coli, where certain plasmids destabilize each other’s inheritance due to competition for the same molecular initiation machinery. By contrast, a model of negative regulation (analogous to the replicon-operator model for transcription) fails to explain the above findings. Nonetheless, research subsequent to Jacob’s, Brenner’s and Cuzin’s proposal of the replicon model has discovered many additional layers of replication control in bacteria and eukaryotes that comprise both positive and negative regulatory elements, highlighting both the complexity and the importance of restricting DNA replication temporally and spatially.
The concept of the replicator as a genetic entity has proven very useful in the quest to identify replicator DNA sequences and initiator proteins in prokaryotes, and to some extent also in eukaryotes, although the organization and complexity of replicators differ considerably between the domains of life. While bacterial genomes typically contain a single replicator that is specified by consensus DNA sequence elements and that controls replication of the entire chromosome, most eukaryotic replicators – with the exception of budding yeast – are not defined at the level of DNA sequence; instead, they appear to be specified combinatorially by local DNA structural and chromatin cues. Eukaryotic chromosomes are also much larger than their bacterial counterparts, raising the need for initiating DNA synthesis from many origins simultaneously to ensure timely replication of the entire genome. Additionally, many more replicative helicases are loaded than activated to initiate replication in a given cell cycle. The context-driven definition of replicators and selection of origins suggests a relaxed replicon model in eukaryotic systems that allows for flexibility in the DNA replication program. Although replicators and origins can be spaced physically apart on chromosomes, they often co-localize or are located in close proximity; for simplicity, we will thus refer to both elements as ‘origins’ throughout this review. Taken together, the discovery and isolation of origin sequences in various organisms represents a significant milestone towards gaining mechanistic understanding of replication initiation. In addition, these accomplishments had profound biotechnological implications for the development of shuttle vectors that can be propagated in bacterial, yeast and mammalian cells.
Bacterial
Most bacterial chromosomes are circular and contain a single origin of chromosomal replication (oriC). Bacterial oriC regions are surprisingly diverse in size (ranging from 250 bp to 2 kbp), sequence, and organization; nonetheless, their ability to drive replication onset typically depends on sequence-specific readout of consensus DNA elements by the bacterial initiator, a protein called DnaA. Origins in bacteria are either continuous or bipartite and contain three functional elements that control origin activity: conserved DNA repeats that are specifically recognized by DnaA (called DnaA-boxes), an AT-rich DNA unwinding element (DUE), and binding sites for proteins that help regulate replication initiation. Interactions of DnaA both with the double-stranded (ds) DnaA-box regions and with single-stranded (ss) DNA in the DUE are important for origin activation and are mediated by different domains in the initiator protein: a Helix-turn-helix (HTH) DNA binding element and an ATPase associated with various cellular activities (AAA+) domain, respectively. While the sequence, number, and arrangement of origin-associated DnaA-boxes vary throughout the bacterial kingdom, their specific positioning and spacing in a given species are critical for oriC function and for productive initiation complex formation.
Among bacteria, E. coli is a particularly powerful model system to study the organization, recognition, and activation mechanism of replication origins. E. coli oriC comprises an approximately ~260 bp region containing four types of initiator binding elements that differ in their affinities for DnaA and their dependencies on the co-factor ATP. DnaA-boxes R1, R2, and R4 constitute high-affinity sites that are bound by the HTH domain of DnaA irrespective of the nucleotide-binding state of the initiator. By contrast, the I, τ, and C-sites, which are interspersed between the R-sites, are low-affinity DnaA-boxes and associate preferentially with ATP-bound DnaA, although ADP-DnaA can substitute for ATP-DnaA under certain conditions. Binding of the HTH domains to the high- and low-affinity DnaA recognition elements promotes ATP-dependent higher-order oligomerization of DnaA’s AAA+ modules into a right-handed filament that wraps duplex DNA around its outer surface, thereby generating superhelical torsion that facilitates melting of the adjacent AT-rich DUE. DNA strand separation is additionally aided by direct interactions of DnaA’s AAA+ ATPase domain with triplet repeats, so-called DnaA-trios, in the proximal DUE region. The engagement of single-stranded trinucleotide segments by the initiator filament stretches DNA and stabilizes the initiation bubble by preventing reannealing. The DnaA-trio origin element is conserved in many bacterial species, indicating it is a key element for origin function. After melting, the DUE provides an entry site for the E. coli replicative helicase DnaB, which is deposited onto each of the single DNA strands by its loader protein DnaC .
Although the different DNA binding activities of DnaA have been extensively studied biochemically and various apo, ssDNA-, or dsDNA-bound structures have been determined, the exact architecture of the higher-order DnaA-oriC initiation assembly remains unclear. Two models have been proposed to explain the organization of essential origin elements and DnaA-mediated oriC melting. The two-state model assumes a continuous DnaA filament that switches from a dsDNA binding mode (the organizing complex) to an ssDNA binding mode in the DUE (the melting complex). By contrast, in the loop-back model, the DNA is sharply bent in oriC and folds back onto the initiator filament so that DnaA protomers simultaneously engage double- and single-stranded DNA regions. Elucidating how exactly oriC DNA is organized by DnaA remains thus an important task for future studies. Insights into initiation complex architecture will help explain not only how origin DNA is melted, but also how a replicative helicase is loaded directionally onto each of the exposed single DNA strands in the unwound DUE, and how these events are aided by interactions of the helicase with the initiator and specific loader proteins.
Archaeal
Archaeal replication origins share some but not all of the organizational features of bacterial oriC. Unlike bacteria, Archaea often initiate replication from multiple origins per chromosome (one to four have been reported); yet, archaeal origins also bear specialized sequence regions that control origin function. These elements include both DNA sequence-specific origin recognition boxes (ORBs or miniORBs) and an AT-rich DUE that is flanked by one or several ORB regions. ORB elements display a considerable degree of diversity in terms of their number, arrangement, and sequence, both among different archaeal species and among different origins within in a single species. An additional degree of complexity is introduced by the initiator, Orc1/Cdc6 in archaea, which binds to ORB regions. Archaeal genomes typically encode multiple paralogs of Orc1/Cdc6 that vary substantially in their affinities for distinct ORB elements and that differentially contribute to origin activities. In Sulfolobus solfataricus, for example, three chromosomal origins have been mapped (oriC1, oriC2, and oriC3), and biochemical studies have revealed complex binding patterns of initiators at these sites. The cognate initiator for oriC1 is Orc1-1, which associates with several ORBs at this origin. OriC2 and oriC3 are bound by both Orc1-1 and Orc1-3. Conversely, a third paralog, Orc1-2, footprints at all three origins but has been postulated to negatively regulate replication initiation. Additionally, the WhiP protein, an initiator unrelated to Orc1/Cdc6, has been shown to bind all origins as well and to drive origin activity of oriC3 in the closely related Sulfolobus islandicus. Because archaeal origins often contain several adjacent ORB elements, multiple Orc1/Cdc6 paralogs can be simultaneously recruited to an origin and oligomerize in some instances; however, in contrast to bacterial DnaA, formation of a higher-order initiator assembly does not appear to be a general prerequisite for origin function in the archaeal domain.
Structural studies have provided insights into how archaeal Orc1/Cdc6 recognizes ORB elements and remodels origin DNA. Orc1/Cdc6 paralogs are two-domain proteins and are composed of a AAA+ ATPase module fused to a C-terminal winged-helix fold. DNA-complexed structures of Orc1/Cdc6 revealed that ORBs are bound by an Orc1/Cdc6 monomer despite the presence of inverted repeat sequences within ORB elements. Both the ATPase and winged-helix regions interact with the DNA duplex but contact the palindromic ORB repeat sequence asymmetrically, which orients Orc1/Cdc6 in a specific direction on the repeat. Interestingly, the DUE-flanking ORB or miniORB elements often have opposite polarities, which predicts that the AAA+ lid subdomains and the winged-helix domains of Orc1/Cdc6 are positioned on either side of the DUE in a manner where they face each other. Since both regions of Orc1/Cdc6 associate with a minichromosome maintenance (MCM) replicative helicase, this specific arrangement of ORB elements and Orc1/Cdc6 is likely important for loading two MCM complexes symmetrically onto the DUE. Surprisingly, while the ORB DNA sequence determines the directionality of Orc1/Cdc6 binding, the initiator makes relatively few sequence-specific contacts with DNA. However, Orc1/Cdc6 severely underwinds and bends DNA, suggesting that it relies on a mix of both DNA sequence and context-dependent DNA structural features to recognize origins. Notably, base pairing is maintained in the distorted DNA duplex upon Orc1/Cdc6 binding in the crystal structures, whereas biochemical studies have yielded contradictory findings as to whether archaeal initiators can melt DNA similarly to bacterial DnaA. Although the evolutionary kinship of archaeal and eukaryotic initiators and replicative helicases indicates that archaeal MCM is likely loaded onto duplex DNA (see next section), the temporal order of origin melting and helicase loading, as well as the mechanism for origin DNA melting, in archaeal systems remains therefore to be clearly established. Likewise, how exactly the MCM helicase is loaded onto DNA needs to be addressed in future studies.
Eukaryotic
Origin organization, specification, and activation in eukaryotes are more complex than in bacterial or archaeal kingdoms and significantly deviate from the paradigm established for prokaryotic replication initiation. The large genome sizes of eukaryotic cells, which range from 12 Mbp in S. cerevisiae to 3 Gbp in humans, necessitates that DNA replication starts at several hundred (in budding yeast) to tens of thousands (in humans) origins to complete DNA replication of all chromosomes during each cell cycle. With the exception of S. cerevisiae and related Saccharomycotina species, eukaryotic origins do not contain consensus DNA sequence elements but their location is influenced by contextual cues such as local DNA topology, DNA structural features, and chromatin environment. Nonetheless, eukaryotic origin function still relies on a conserved initiator protein complex to load replicative helicases onto DNA during the late M and G1 phases of the cell cycle, a step known as origin licensing. In contrast to their bacterial counterparts, replicative helicases in eukaryotes are loaded onto origin duplex DNA in an inactive, double-hexameric form and only a subset of them (10-20% in mammalian cells) is activated during any given S phase, events that are referred to as origin firing. The location of active eukaryotic origins is therefore determined on at least two different levels, origin licensing to mark all potential origins, and origin firing to select a subset that permits assembly of the replication machinery and initiation of DNA synthesis. The extra licensed origins serve as backup and are activated only upon slowing or stalling of nearby replication forks, ensuring that DNA replication can be completed when cells encounter replication stress. Together, the excess of licensed origins and the tight cell cycle control of origin licensing and firing embody two important strategies to prevent under- and overreplication and to maintain the integrity of eukaryotic genomes.
Early studies in S. cerevisiae indicated that replication origins in eukaryotes might be recognized in a DNA-sequence-specific manner analogously to those in prokaryotes. In budding yeast, the search for genetic replicators lead to the identification of autonomously replicating sequences (ARS) that support efficient DNA replication initiation of extrachromosomal DNA. These ARS regions are approximately 100-200 bp long and exhibit a multipartite organization, containing A, B1, B2, and sometimes B3 elements that together are essential for origin function. The A element encompasses the conserved 11 bp ARS consensus sequence (ACS), which, in conjunction with the B1 element, constitutes the primary binding site for the heterohexameric origin recognition complex (ORC), the eukaryotic replication initiator. Within ORC, five subunits are predicated on conserved AAA+ ATPase and winged-helix folds and co-assemble into a pentameric ring that encircles DNA. In budding yeast ORC, DNA binding elements in the ATPase and winged-helix domains, as well as adjacent basic patch regions in some of the ORC subunits, are positioned in the central pore of the ORC ring such that they aid the DNA-sequence-specific recognition of the ACS in an ATP-dependent manner. By contrast, the roles of the B2 and B3 elements are less clear. The B2 region is similar to the ACS in sequence and has been suggested to function as a second ORC binding site under certain conditions, or as a binding site for the replicative helicase core. Conversely, the B3 element recruits the transcription factor Abf1, albeit B3 is not found at all budding yeast origins and Abf1 binding does not appear to be strictly essential for origin function.
Origin recognition in eukaryotes other than S. cerevisiae or its close relatives does not conform to the sequence-specific read-out of conserved origin DNA elements. Pursuits to isolate specific chromosomal replicator sequences more generally in eukaryotic species, either genetically or by genome-wide mapping of initiator binding or replication start sites, have failed to identify clear consensus sequences at origins. Thus, sequence-specific DNA-initiator interactions in budding yeast signify a specialized mode for origin recognition in this system rather than an archetypal mode for origin specification across the eukaryotic domain. Nonetheless, DNA replication does initiate at discrete sites that are not randomly distributed across eukaryotic genomes, arguing that alternative means determine the chromosomal location of origins in these systems. These mechanisms involve a complex interplay between DNA accessibility, nucleotide sequence skew (both AT-richness and CpG islands have been linked to origins), Nucleosome positioning, epigenetic features, DNA topology and certain DNA structural features (e.g., G4 motifs), as well as regulatory proteins and transcriptional interference. Importantly, origin properties vary not only between different origins in an organism and among species, but some can also change during development and cell differentiation. The chorion locus in Drosophila follicle cells constitutes a well-established example for spatial and developmental control of initiation events. This region undergoes DNA-replication-dependent gene amplification at a defined stage during oogenesis and relies on the timely and specific activation of chorion origins, which in turn is regulated by origin-specific cis-elements and several protein factors, including the Myb complex, E2F1, and E2F2. This combinatorial specification and multifactorial regulation of metazoan origins has complicated the identification of unifying features that determine the location of replication start sites across eukaryotes more generally.
To facilitate replication initiation and origin recognition, ORC assemblies from various species have evolved specialized auxiliary domains that are thought to aid initiator targeting to chromosomal origins or chromosomes in general. For example, the Orc4 subunit in S. pombe ORC contains several AT-hooks that preferentially bind AT-rich DNA, while in metazoan ORC the TFIIB-like domain of Orc6 is thought to perform a similar function. Metazoan Orc1 proteins also harbor a bromo-adjacent homology (BAH) domain that interacts with H4K20me2-nucleosomes. Particularly in mammalian cells, H4K20 methylation has been reported to be required for efficient replication initiation, and the Orc1-BAH domain facilitates ORC association with chromosomes and Epstein-Barr virus origin-dependent replication. Therefore, it is intriguing to speculate that both observations are mechanistically linked at least in a subset of metazoa, but this possibility needs to be further explored in future studies. In addition to the recognition of certain DNA or epigenetic features, ORC also associates directly or indirectly with several partner proteins that could aid initiator recruitment, including LRWD1, PHIP (or DCAF14), HMGA1a, among others. Interestingly, Drosophila ORC, like its budding yeast counterpart, bends DNA and negative supercoiling has been reported to enhance DNA binding of this complex, suggesting that DNA shape and malleability might influence the location of ORC binding sites across metazoan genomes. A molecular understanding for how ORC’s DNA binding regions might support the read out of structural properties of the DNA duplex in metazoans rather than of specific DNA sequences as in S. cerevisiae awaits high-resolution structural information of DNA-bound metazoan initiator assemblies. Likewise, whether and how different epigenetic factors contribute to initiator recruitment in metazoan systems is poorly defined and is an important question that needs to be addressed in more detail.
Once recruited to origins, ORC and its co-factors Cdc6 and Cdt1 drive the deposition of the minichromosome maintenance 2-7 (Mcm2-7) complex onto DNA. Like the archaeal replicative helicase core, Mcm2-7 is loaded as a head-to-head double hexamer onto DNA to license origins. In S-phase, Dbf4-dependent kinase (DDK) and Cyclin-dependent kinase (CDK) phosphorylate several Mcm2-7 subunits and additional initiation factors to promote the recruitment of the helicase co-activators Cdc45 and GINS, DNA melting, and ultimately bidirectional replisome assembly at a subset of the licensed origins. In both yeast and metazoans, origins are free or depleted of nucleosomes, a property that is crucial for Mcm2-7 loading, indicating that chromatin state at origins regulates not only initiator recruitment but also helicase loading. A permissive chromatin environment is further important for origin activation and has been implicated in regulating both origin efficiency and the timing of origin firing. Euchromatic origins typically contain active chromatin marks, replicate early, and are more efficient than late-replicating, heterochromatic origins, which conversely are characterized by repressive marks. Not surprisingly, several chromatin remodelers and chromatin-modifying enzymes have been found to associate with origins and certain initiation factors, but how their activities impact different replication initiation events remains largely obscure. Remarkably, cis-acting “early replication control elements” (ECREs) have recently also been identified to help regulate replication timing and to influence 3D genome architecture in mammalian cells. Understanding the molecular and biochemical mechanisms that orchestrate this complex interplay between 3D genome organization, local and higher-order chromatin structure, and replication initiation is an exciting topic for further studies.
Why have metazoan replication origins diverged from the DNA sequence-specific recognition paradigm that determines replication start sites in prokaryotes and budding yeast? Observations that metazoan origins often co-localize with promoter regions in Drosophila and mammalian cells and that replication-transcription conflicts due to collisions of the underlying molecular machineries can lead to DNA damage suggest that proper coordination of transcription and replication is important for maintaining genome stability. Recent findings also point to a more direct role of transcription in influencing the location of origins, either by inhibiting Mcm2-7 loading or by repositioning of loaded Mcm2-7 on chromosomes. Sequence-independent (but not necessarily random) initiator binding to DNA additionally allows for flexibility in specifying helicase loading sites and, together with transcriptional interference and the variability in activation efficiencies of licensed origins, likely determines origin location and contributes to the co-regulation of DNA replication and transcriptional programs during development and cell fate transitions. Computational modeling of initiation events in S. pombe, as well as the identification of cell-type specific and developmentally-regulated origins in metazoans, are in agreement with this notion. However, a large degree of flexibility in origin choice also exists among different cells within a single population, albeit the molecular mechanisms that lead to the heterogeneity in origin usage remain ill-defined. Mapping origins in single cells in metazoan systems and correlating these initiation events with single-cell gene expression and chromatin status will be important to elucidate whether origin choice is purely stochastic or controlled in a defined manner.
Viral
Viruses often possess a single origin of replication.
A variety of proteins have been described as being involved in viral replication. For instance, Polyoma viruses utilize host cell DNA polymerases, which attach to a viral origin of replication if the T antigen is present.
Variations
Although DNA replication is essential for genetic inheritance, defined, site-specific replication origins are technically not a requirement for genome duplication as long as all chromosomes are copied in their entirety to maintain gene copy numbers. Certain bacteriophages and viruses, for example, can initiate DNA replication by homologous recombination independent of dedicated origins. Likewise, the archaeon Haloferax volcanii uses recombination-dependent initiation to duplicate its genome when its endogenous origins are deleted. Similar non-canonical initiation events through break-induced or transcription-initiated replication have been reported in E. coli and S. cerevisiae. Nonetheless, despite the ability of cells to sustain viability under these exceptional circumstances, origin-dependent initiation is a common strategy universally adopted across different domains of life.
In addition, detailed studies of replication initiation have focused on a limited number of model systems. The extensively studied fungi and metazoa are both members of the opisthokont supergroup and exemplify only a small fraction of the evolutionary landscape in the eukaryotic domain. Comparably few efforts have been directed at other eukaryotic model systems, such as kinetoplastids or tetrahymena. Surprisingly, these studies have revealed interesting differences both in origin properties and in initiator composition compared to yeast and metazoans.
See also
References
A wikidata item for the academic article needs to be provided (search Wikidata). See template documentation for details.)
- Technical Glossary Edward K. Wagner, Martinez Hewlett, David Bloom and David Camerini Basic Virology Third Edition, Blackwell publishing, 2007 ISBN 1-4051-4715-6
- Hulo C, de Castro E, Masson P, Bougueleret L, Bairoch A, Xenarios I, Le Mercier P (January 2011). "ViralZone: a knowledge resource to understand virus diversity". Nucleic Acids Research. 39 (Database issue): D576-82. doi:10.1093/nar/gkq901. PMC 3013774. PMID 20947564.
- Mendel, J.G. (1866). "Versuche über Pflanzenhybriden", Verhandlungen des naturforschenden Vereines in Brünn, Bd. IV für das Jahr, 1865, Abhandlungen: 3–47. For the English translation, see: Druery, C.T.; Bateson, William (1901). "Experiments in plant hybridization" (PDF). Journal of the Royal Horticultural Society. 26: 1–32. Retrieved 9 October 2009.
- Avery, O. T.; Macleod, C. M.; McCarty, M. (1944-02-01). "STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES : INDUCTION OF TRANSFORMATION BY A DESOXYRIBONUCLEIC ACID FRACTION ISOLATED FROM PNEUMOCOCCUS TYPE III". The Journal of Experimental Medicine. 79 (2): 137–158. doi:10.1084/jem.79.2.137. ISSN 0022-1007. PMC 2135445. PMID 19871359.
- Watson, J. D.; Crick, F. H. (1953). "The structure of DNA". Cold Spring Harbor Symposia on Quantitative Biology. 18: 123–131. doi:10.1101/sqb.1953.018.01.020. ISSN 0091-7451. PMID 13168976.
- Meselson, M.; Stahl, F. W. (1958-07-15). "THE REPLICATION OF DNA IN ESCHERICHIA COLI". Proceedings of the National Academy of Sciences of the United States of America. 44 (7): 671–682. doi:10.1073/pnas.44.7.671. ISSN 0027-8424. PMC 528642. PMID 16590258.
{{cite journal}}
: CS1 maint: PMC format (link) - Meselson, M.; Stahl, F. W. (1958). "The replication of DNA". Cold Spring Harbor Symposia on Quantitative Biology. 23: 9–12. doi:10.1101/sqb.1958.023.01.004. ISSN 0091-7451. PMID 13635537.
- Lehman, I. R.; Bessman, M. J.; Simms, E. S.; Kornberg, A. (1958). "Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli". The Journal of Biological Chemistry. 233 (1): 163–170. ISSN 0021-9258. PMID 13563462.
- O'Donnell, Michael; Langston, Lance; Stillman, Bruce (2013-07-01). "Principles and concepts of DNA replication in bacteria, archaea, and eukarya". Cold Spring Harbor Perspectives in Biology. 5 (7). doi:10.1101/cshperspect.a010108. ISSN 1943-0264. PMC 3685895. PMID 23818497.
- Abbas, Tarek; Keaton, Mignon A.; Dutta, Anindya (2013-03-01). "Genomic instability in cancer". Cold Spring Harbor Perspectives in Biology. 5 (3): a012914. doi:10.1101/cshperspect.a012914. ISSN 1943-0264. PMC 3578360. PMID 23335075.
- ^ Barlow, Jacqueline H.; Nussenzweig, André (2014). "Replication initiation and genome instability: a crossroads for DNA and RNA synthesis". Cellular and molecular life sciences: CMLS. 71 (23): 4545–4559. doi:10.1007/s00018-014-1721-1. ISSN 1420-9071. PMC 6289259. PMID 25238783.
- Siddiqui, Khalid; On, Kin Fan; Diffley, John F. X. (2013-09-01). "Regulating DNA replication in eukarya". Cold Spring Harbor Perspectives in Biology. 5 (9). doi:10.1101/cshperspect.a012930. ISSN 1943-0264. PMC 3753713. PMID 23838438.
- Sclafani, R. A.; Holzen, T. M. (2007). "Cell cycle regulation of DNA replication". Annual Review of Genetics. 41: 237–280. doi:10.1146/annurev.genet.41.110306.130308. ISSN 0066-4197. PMC 2292467. PMID 17630848.
- ^ García-Muse, Tatiana; Aguilera, Andrés (2016). "Transcription-replication conflicts: how they occur and how they are resolved". Nature Reviews. Molecular Cell Biology. 17 (9): 553–563. doi:10.1038/nrm.2016.88. ISSN 1471-0080. PMID 27435505.
- Yakovchuk P, Protozanova E, Frank-Kamenetskii MD (2006). "Base-stacking and base-pairing contributions into thermal stability of the DNA double helix". Nucleic Acids Research. 34 (2): 564–74. doi:10.1093/nar/gkj454. PMC 1360284. PMID 16449200.
- Leonard, Alan C.; Méchali, Marcel (2013-10-01). "DNA replication origins". Cold Spring Harbor Perspectives in Biology. 5 (10): a010116. doi:10.1101/cshperspect.a010116. ISSN 1943-0264. PMC 3783049. PMID 23838439.
- Creager, Rachel L.; Li, Yulong; MacAlpine, David M. (2015-04-09). "SnapShot: Origins of DNA replication". Cell. 161 (2): 418–418.e1. doi:10.1016/j.cell.2015.03.043. ISSN 1097-4172. PMID 25860614.
- Knott, Simon R. V.; Viggiani, Christopher J.; Aparicio, Oscar M. (2009-08-16). "To promote and protect: coordinating DNA replication and transcription for genome stability". Epigenetics. 4 (6): 362–365. doi:10.4161/epi.4.6.9712. ISSN 1559-2308. PMID 19736523.
- Deshpande, A. M.; Newlon, C. S. (1996-05-17). "DNA replication fork pause sites dependent on transcription". Science (New York, N.Y.). 272 (5264): 1030–1033. doi:10.1126/science.272.5264.1030. ISSN 0036-8075. PMID 8638128.
- Sankar, T. Sabari; Wastuwidyaningtyas, Brigitta D.; Dong, Yuexin; Lewis, Sarah A.; Wang, Jue D. (2016). "The nature of mutations induced by replication–transcription collisions". Nature. 535 (7610): 178–181. doi:10.1038/nature18316. ISSN 1476-4687. PMC 4945378. PMID 27362223.
- Liu, B.; Alberts, B. M. (1995-02-24). "Head-on collision between a DNA replication apparatus and RNA polymerase transcription complex". Science (New York, N.Y.). 267 (5201): 1131–1137. doi:10.1126/science.7855590. ISSN 0036-8075. PMID 7855590.
- Azvolinsky, Anna; Giresi, Paul G.; Lieb, Jason D.; Zakian, Virginia A. (2009-06-26). "Highly transcribed RNA polymerase II genes are impediments to replication fork progression in Saccharomyces cerevisiae". Molecular Cell. 34 (6): 722–734. doi:10.1016/j.molcel.2009.05.022. ISSN 1097-4164. PMC 2728070. PMID 19560424.
- ^ Jacob, F.; Brenner, S.; Cuzin, F. (1963-01-01). "On the Regulation of DNA Replication in Bacteria". Cold Spring Harbor Symposia on Quantitative Biology. 28 (0): 329–348. doi:10.1101/sqb.1963.028.01.048. ISSN 0091-7451.
- Novick, R. P. (1987). "Plasmid incompatibility". Microbiological Reviews. 51 (4): 381–395. ISSN 0146-0749. PMC 373122. PMID 3325793.
{{cite journal}}
: CS1 maint: PMC format (link) - Skarstad, Kirsten; Katayama, Tsutomu (2013-04-01). "Regulating DNA replication in bacteria". Cold Spring Harbor Perspectives in Biology. 5 (4): a012922. doi:10.1101/cshperspect.a012922. ISSN 1943-0264. PMC 3683904. PMID 23471435.
- ^ Marks, Anna B.; Fu, Haiqing; Aladjem, Mirit I. (2017). "Regulation of Replication Origins". Advances in Experimental Medicine and Biology. 1042: 43–59. doi:10.1007/978-981-10-6955-0_2. ISSN 0065-2598. PMC 6622447. PMID 29357052.
- ^ Parker, Matthew W.; Botchan, Michael R.; Berger, James M. (2017). "Mechanisms and regulation of DNA replication initiation in eukaryotes". Critical Reviews in Biochemistry and Molecular Biology. 52 (2): 107–144. doi:10.1080/10409238.2016.1274717. ISSN 1549-7798. PMC 5545932. PMID 28094588.
- ^ Gilbert, David M. (2004). "In search of the holy replicator". Nature Reviews. Molecular Cell Biology. 5 (10): 848–855. doi:10.1038/nrm1495. ISSN 1471-0072. PMC 1255919. PMID 15459665.
- Aladjem, Mirit I.; Fanning, Ellen (2004-7). "The replicon revisited: an old model learns new tricks in metazoan chromosomes". EMBO reports. 5 (7): 686–691. doi:10.1038/sj.embor.7400185. ISSN 1469-221X. PMC 1299096. PMID 15229645.
{{cite journal}}
: Check date values in:|date=
(help) - ^ Remus, Dirk; Beall, Eileen L.; Botchan, Michael R. (2004-02-25). "DNA topology, not DNA sequence, is a critical determinant for Drosophila ORC-DNA binding". The EMBO journal. 23 (4): 897–907. doi:10.1038/sj.emboj.7600077. ISSN 0261-4189. PMC 380993. PMID 14765124.
{{cite journal}}
: CS1 maint: PMC format (link) - Vashee, Sanjay; Cvetic, Christin; Lu, Wenyan; Simancek, Pamela; Kelly, Thomas J.; Walter, Johannes C. (2003-08-01). "Sequence-independent DNA binding and replication initiation by the human origin recognition complex". Genes & Development. 17 (15): 1894–1908. doi:10.1101/gad.1084203. ISSN 0890-9369. PMC 196240. PMID 12897055.
{{cite journal}}
: CS1 maint: PMC format (link) - ^ Shen, Zhen; Sathyan, Kizhakke M.; Geng, Yijie; Zheng, Ruiping; Chakraborty, Arindam; Freeman, Brian; Wang, Fei; Prasanth, Kannanganattu V.; Prasanth, Supriya G. (2010-10-08). "A WD-repeat protein stabilizes ORC binding to chromatin". Molecular Cell. 40 (1): 99–111. doi:10.1016/j.molcel.2010.09.021. ISSN 1097-4164. PMC 5201136. PMID 20932478.
- ^ Dorn, Elizabeth Suzanne; Cook, Jeanette Gowen (2011). "Nucleosomes in the neighborhood: new roles for chromatin modifications in replication origin control". Epigenetics. 6 (5): 552–559. doi:10.4161/epi.6.5.15082. ISSN 1559-2308. PMC 3230546. PMID 21364325.
- ^ Aladjem, Mirit I.; Redon, Christophe E. (2017). "Order from clutter: selective interactions at mammalian replication origins". Nature Reviews. Genetics. 18 (2): 101–116. doi:10.1038/nrg.2016.141. ISSN 1471-0064. PMC 6596300. PMID 27867195.
- ^ Fragkos, Michalis; Ganier, Olivier; Coulombe, Philippe; Méchali, Marcel (2015). "DNA replication origin activation in space and time". Nature Reviews. Molecular Cell Biology. 16 (6): 360–374. doi:10.1038/nrm4002. ISSN 1471-0080. PMID 25999062.
- ^ Prioleau, Marie-Noëlle; MacAlpine, David M. (2016). "DNA replication origins-where do we begin?". Genes & Development. 30 (15): 1683–1697. doi:10.1101/gad.285114.116. ISSN 1549-5477. PMC 5002974. PMID 27542827.
- Cayrou, Christelle; Coulombe, Philippe; Puy, Aurore; Rialle, Stephanie; Kaplan, Noam; Segal, Eran; Méchali, Marcel (2012-02-15). "New insights into replication origin characteristics in metazoans". Cell Cycle (Georgetown, Tex.). 11 (4): 658–667. doi:10.4161/cc.11.4.19097. ISSN 1551-4005. PMC 3318102. PMID 22373526.
- Lombraña, Rodrigo; Almeida, Ricardo; Álvarez, Alba; Gómez, María (2015). "R-loops and initiation of DNA replication in human cells: a missing link?". Frontiers in Genetics. 6: 158. doi:10.3389/fgene.2015.00158. ISSN 1664-8021. PMC 4412123. PMID 25972891.
{{cite journal}}
: CS1 maint: unflagged free DOI (link) - Jang, Sang-Min; Zhang, Ya; Utani, Koichi; Fu, Haiqing; Redon, Christophe E.; Marks, Anna B.; Smith, Owen K.; Redmond, Catherine J.; Baris, Adrian M. (2018). "The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin". Nature Communications. 9 (1): 2782. doi:10.1038/s41467-018-05177-6. ISSN 2041-1723. PMC 6050238. PMID 30018425.
- Zakian, V. A.; Scott, J. F. (1982). "Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived from Saccharomyces cerevisiae chromosomal DNA". Molecular and Cellular Biology. 2 (3): 221–232. doi:10.1128/mcb.2.3.221. ISSN 0270-7306. PMC 369780. PMID 6287231.
{{cite journal}}
: CS1 maint: PMC format (link) - Rhodes, N.; Company, M.; Errede, B. (1990). "A yeast-Escherichia coli shuttle vector containing the M13 origin of replication". Plasmid. 23 (2): 159–162. ISSN 0147-619X. PMID 2194231.
{{cite journal}}
:|last2=
has generic name (help) - Paululat, Achim; Heinisch, Jürgen J. (2012-12-15). "New yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructs". Gene. 511 (2): 300–305. doi:10.1016/j.gene.2012.09.058. ISSN 1879-0038. PMID 23026211.
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- Lewin, Benjamin (2004). Genes VIII. Prentice Hall.
External links
- Ori-Finder, an online software for prediction of bacterial and archaeal oriCs
- Replication+Origin at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
DNA replication (comparing prokaryotic to eukaryotic) | |||||||
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Initiation |
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Replication |
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Termination |