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ELISA

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The Enzyme Linked Immuno-Sorbent Assay or ELISA is a method commonly employed in biochemistry to detect if a certain protein is present in a given sample. The steps in this procedure are:

  1. Create an antibody which specifically binds to the protein of interest.
  2. Attach this antibody to a solid support (usually a membrane) .
  3. Apply the sample to this membrane, and let the protein bind to the antibody.
  4. Wash the membrane, so that only the protein bound to the antibody remains.
  5. Apply a second antibody which is linked to an enzyme and binds to the proteine of interest.
  6. Apply a chemical which is coverted by the enzyme into a flourescent signal.
  7. View the result

Any basic immunology or molecular biology textbook should have more information. Try http://www.ncbi.nlm.nih.gov. They got books.