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Hemolysis assay
The hemolysis assay is a practical scalable assay for the initial assessment of cytotoxicity in drug development.
dinQ
dinQ, DNA-damage-inducible protein Q produces two major transcripts, only one, however, +44, is actively translated. Translation is initiated using the unusual GUG start codon and results in a 27 amino acid peptide. Plasmid-based expression of C-terminal triple FLAG-tagged dinQ located DinQ in the inner-membrane of E. coli. Overexpression of dinQ led to reduced survival, loss of transmembrane electrical polarity and reduced intracellular ATP concentrations.
- Sæbø PI, Bjørås M, Franzyk H, Helgensen E, Booth J (February 2, 2023). "Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity". Int. J. Mol. Sci. 24 (3). doi:10.3390/ijms24032914. PMC 9917735. PMID 36769243.