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Since there is only one NCAA championship for women's water polo, all women's water polo programs, whether from Division I, Division II, or Division III, are eligible. For the first time, ten teams participated in the tournament. The automatic qualifiers came from the following conferences: Big West Conference, Collegiate Water Polo Association, Golden Coast Conference, Metro Atlantic Athletic Conference, Mountain Pacific Sports Federation, Southern California Intercollegiate Athletic Conference, and Western Water Polo Association. Three additional teams were selected at large without geographical restrictions by the selection committee. The four lowest seeded teams played in the two play-in games on Tuesday, May 10.
