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25-hydroxycholesterol 7alpha-hydroxylase (EC1.14.13.100, 25-hydroxycholesterol 7alpha-monooxygenase, CYP7B1, CYP7B1 oxysterol 7alpha-hydroxylase) is an enzyme with systematic namecholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating). This enzyme catalyses the following chemical reaction
Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Björkhem I (September 1994). "7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase". European Journal of Biochemistry. 224 (2): 309–16. doi:10.1111/j.1432-1033.1994.00309.x. PMID7925343.