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3-Amino-9-ethylcarbazole

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3-Amino-9-ethylcarbazole
Names
Preferred IUPAC name 9-Ethyl-9H-carbazol-3-amine
Other names AEC; 9-Ethylcarbazol-3-amine
Identifiers
CAS Number
3D model (JSmol)
ChEBI
ChEMBL
ChemSpider
ECHA InfoCard 100.004.599 Edit this at Wikidata
EC Number
  • 205-057-7
MeSH 3-amino-9-ethylcarbazole
PubChem CID
UNII
UN number 2811
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3Key: OXEUETBFKVCRNP-UHFFFAOYSA-N
SMILES
  • CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31
Properties
Chemical formula C14H14N2
Molar mass 210.280 g·mol
Hazards
Occupational safety and health (OHS/OSH):
Main hazards Acutely toxic, Health hazards
GHS labelling:
Pictograms GHS08: Health hazard
Signal word Danger
Hazard statements H350
Precautionary statements P201, P202, P281, P308+P313, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). ☒N (what is  ?) Infobox references
Chemical compound

3-Amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry, specifically for visualizing sections stained with HRP-conjugated secondary antibodies. After the chromogenic oxidation reaction catalyzed by HRP, a red water-insoluble precipitate is formed in situ, visualizing the location of the antigen detected by the HRP-conjugated antibody. The resulting stained section can be destained by organic solvents in which the red precipitate is soluble.

References

  1. Edward C., Stack; Chichung, Wang; Kristin A., Roman; Clifford C., Hoyt (2014). "Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis". Methods. 70 (1): 46–58. doi:10.1016/j.ymeth.2014.08.016. PMID 25242720.
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