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3-Isopropylmalate dehydrogenase (EC 1.1.1.85) is an enzyme that is a part of the isopropylmalate dehydrogenase family, which catalyzes the chemical reactions:

(2R,3S)-3-isopropylmalate + NAD ${\displaystyle \rightleftharpoons }$ 4-methyl-2-oxopentanoate + CO₂ + NADH

(2R,3S)-3-isopropylmalate + NAD ${\displaystyle \rightleftharpoons }$ (2S)-2-isopropyl-3-oxosuccinate + H + NADH

(2S)-2-isopropyl-3-oxosuccinate + H ${\displaystyle \rightleftharpoons }$ 4-methyl-2-oxopentanoate + CO₂

References

1. Burns RO, Umbarger HE, Gross SR (1963). "The biosynthesis of leucine. III. The conversion of α-hydroxy-β-carboxyisocaproate to α-ketoisocaproate". Biochemistry. 2 (5): 1053–8. doi:10.1021/bi00905a024. PMID 14087358.
2. Calvo JM, Stevens CM, Kalyanpur MG, Umbarger HE (December 1964). "The Absolute Configuration of α-carboxyisocaproic Acid (3-Isopropylmalic Acid), an Intermediate in Leucine Biosynthesis". Biochemistry. 3 (12): 2024–7. doi:10.1021/bi00900a043. PMID 14269331.
3. Parsons SJ, Burns RO (February 1969). "Purification and Properties of β-Isopropylmalate Dehydrogenase". J. Biol. Chem. 244 (3): 996–1003. doi:10.1016/S0021-9258(18)91884-3. PMID 4889950.
4. Németh A, Svingor A, Pócsik M, Dobó J, Magyar C, Szilágyi A, Gál P, Závodszky P (February 2000). "Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase". FEBS Lett. 468 (1): 48–52. Bibcode:2000FEBSL.468...48N. doi:10.1016/S0014-5793(00)01190-X. PMID 10683439. S2CID 44544921.

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