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In enzymology, a 4-oxoproline reductase (EC 1.1.1.104) is an enzyme that catalyzes the chemical reaction

4-oxo-L-proline + NADH + H ${\displaystyle \rightleftharpoons }$ cis-4-hydroxy-L-proline + NAD

Thus, the three substrates of this enzyme are 4-oxo-L-proline, NADH, and H, whereas its two products are cis-4-hydroxy-L-proline and NAD.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD or NADP as acceptor. The systematic name of this enzyme class is 4-hydroxy-L-proline:NAD oxidoreductase. This enzyme is also called hydroxy-L-proline oxidase. This enzyme was originally thought to participate in the metabolism of arginine and proline. However, recent data show that it is unlikely since neither 4-oxo-L-proline nor cis-4-hydroxy-L-proline are metabolites of these metabolic pathways.

Gene

The gene encoding 4-oxo-L-proline reductase was identified as 3-hydroxybutyrate dehydrogenase 2 (BDH2) by Sebastian Kwiatkowski and co-workers in 2022. The enzyme is a member of the Short-chain Dehydrogenases/Reductases (SDR) family of enzymes.

References

1. ^ Kwiatkowski S, Bozko M, Zarod M, Witecka A, Kocdemir K, Jagielski AK, Drozak J (February 2022). "Recharacterization of the Mammalian Cytosolic Type 2 (R)-β-Hydroxybutyrate Dehydrogenase (BDH2) as 4-Oxo-L-Proline Reductase (EC 1.1.1.104)". The Journal of Biological Chemistry: 101708. doi:10.1016/j.jbc.2022.101708. PMC 8914325. PMID 35150746.

Further reading

• Smith TE, Mitoma C (April 1962). "Partial purification and some properties of 4-ketoproline reductase". The Journal of Biological Chemistry. 237: 1177–1180. PMID 13914427.

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