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In enzymology, a 5-oxoprolinase (ATP-hydrolysing) (EC 3.5.2.9) is an enzyme that catalyzes the chemical reaction

ATP + 5-oxo-L-proline + 2 H₂O ${\displaystyle \rightleftharpoons }$ ADP + phosphate + L-glutamate

The 3 substrates of this enzyme are ATP, 5-oxo-L-proline, and H₂O, whereas its 3 products are ADP, phosphate, and L-glutamate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-oxo-L-proline amidohydrolase (ATP-hydrolysing). Other names in common use include pyroglutamase (ATP-hydrolysing), oxoprolinase, pyroglutamase, 5-oxoprolinase, pyroglutamate hydrolase, pyroglutamic hydrolase, L-pyroglutamate hydrolase, 5-oxo-L-prolinase, and pyroglutamase. This enzyme participates in glutathione metabolism.

References

• Van der Werf P, Orlowski M, Meister A (1971). "Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle". Proc. Natl. Acad. Sci. U.S.A. 68 (12): 2982–5. doi:10.1073/pnas.68.12.2982. PMC 389574. PMID 5289242.

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