A66, A 66 or A-66 may refer to:
- A66 State Route (Australia), a road in South Australia connecting Keith and Port MacDonnell
- Autopista 66 (Cuba), motorway connecting the Autopista Nacional with Sancti Spiritus
- A66 road (England), a road connecting Middlesbrough and Penrith/Workington
- A66 motorway (France), a road connecting Villefranche-de-Lauragais and Pamiers
- A66 motorway (Germany), a road connecting the Taunus and Fulda
- A66 motorway (Spain), a road connecting Gijón and Seville
- Benoni Defense, in the Encyclopaedia of Chess Openings
- HLA-A66, an HLA-A serotype
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