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Beacon Designer designs primers and probes for real-time PCR (polymerase chain reaction) assays. It is compatible to work on Windows as well as on Mac. The software currently supports the following real-time PCR chemistries for primer and probe design:

• SYBR Green
• TaqMan
• Molecular beacon
• HRMA Primers
• Scorpions
• FRET
• LNA (Locked nucleic acid)
• NASBA

Beacon Designer program has built-in support for homology avoidance using the standard web BLAST service or the set-up of a desktop BLAST feature for the identification of regions that are significantly cross-homologous with the target gene sequence. The software designs real-time PCR primers and probes for complex differential gene expression and allele discrimination assays.

Beacon Designer has been used in workshops and conferences related to real-time PCR study as a reference software.

External links

• High-Resolution Melt

• Bioinformatics software