Misplaced Pages

CUT&Tag sequencing

Article snapshot taken from Wikipedia with creative commons attribution-sharealike license. Give it a read and then ask your questions in the chat. We can research this topic together.
Method used to analyze protein interactions with DNA

CUT&Tag-sequencing, also known as cleavage under targets and tagmentation, is a method used to analyze protein interactions with DNA. CUT&Tag-sequencing combines antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global DNA binding sites precisely for any protein of interest. Currently, ChIP-Seq is the most common technique utilized to study protein–DNA relations, however, it suffers from a number of practical and economical limitations that CUT&RUN and CUT&Tag sequencing do not. CUT&Tag sequencing is an improvement over CUT&RUN because it does not require cells to be lysed or chromatin to be fractionated. CUT&RUN is not suitable for single-cell platforms so CUT&Tag is advantageous for these.

Uses

CUT&Tag-sequencing can be used to examine gene regulation or to analyze transcription factor and other chromatin-associated protein binding. Protein-DNA interactions regulate gene expression and are responsible for many biological processes and disease states. This epigenetic information is complementary to genotype and expression analysis. CUT&Tag is an alternative to the current standard of ChIP-seq. ChIP-Seq suffers from limitations due to the cross linking step in ChIP-Seq protocols that can promote epitope masking and generate false-positive binding sites. As well, ChIP-seq suffers from suboptimal signal-to-noise ratios and poor resolution. CUT&Run-sequencing and CUT&Tag have the advantage of being simpler techniques with lower costs due to the high signal-to-noise ratio, requiring less depth in sequencing.

Specific DNA sites in direct physical interaction with proteins such as transcription factors can be isolated by Protein-A (pA) conjugated Tn5 bound to a protein of interest. Tn5 mediated cleavage produces a library of target DNA sites bound to a protein of interest in situ. Sequencing of prepared DNA libraries and comparison to whole-genome sequence databases allows researchers to analyze the interactions between target proteins and DNA, as well as differences in epigenetic chromatin modifications. Therefore, the CUT&Tag method may be applied to proteins and modifications, including transcription factors, polymerases, structural proteins, protein modifications, and DNA modifications.

Sequencing

Unlike ChIP-Seq there is no size selection required before sequencing. A single sequencing run can scan for genome-wide associations with high resolution, due to the low background achieved by performing the reaction in situ with the CUT&RUN-sequencing methodology. ChIP-Seq, by contrast, requires ten times the sequencing depth because of the intrinsically high background associated with the method. The data is then collected and analyzed using software that aligns sample sequences to a known genomic sequence to identify the CUT&Tag DNA fragments.

Protocols

There are detailed CUT&Tag workflows available in an open-access methods repository.

Sensitivity

CUT&Run-Sequencing or CUT&Tag-Sequencing provide low levels of background signal because of in situ profiling which retains in vivo 3D confirmations of transcription factor-DNA interactions, so antibodies access only exposed surfaces. Sensitivity of sequencing depends on the depth of the sequencing run (i.e. the number of mapped sequence tags), the size of the genome and the distribution of the target factor. The sequencing depth is directly correlated with cost and negatively correlated with background. Therefore, low-background CUT&Tag sequencing is inherently more cost-effective than high-background ChIP-Sequencing.

Peak calling representation for H3K27me3 targeted sequencing results, comparing CUT&RUN to traditional ChIP. Note That CUT&RUN and CUT&Tag appears to deliver improved signal-to-noise ratio than traditional ChIP. This advantage translates to lower sequencing costs (and feasibility in single-cells for CUT&Tag).

Limitations

The primary limitation of CUT&Tag-seq is the likelihood of over-digestion of DNA due to inappropriate timing of the Magnesium-dependent Tn5 reaction. A similar limitation exists for contemporary ChIP-Seq protocols where enzymatic or sonicated DNA shearing must be optimized. As with ChIP-Seq, a good quality antibody targeting the protein of interest is required. As with other techniques using Tn5, the library preparation has a strong GC bias and has poor sensitivity in low GC regions or genomes with high variance in GC content.

Similar methods

  • Sono-Seq: Identical to ChIP-Seq but without the immunoprecipitation step.
  • HITS-CLIP: Also called CLIP-Seq, employed to detect interactions with RNA rather than DNA.
  • PAR-CLIP: A method for identifying the binding sites of cellular RNA-binding proteins.
  • RIP-Chip: Similar to ChIP-Seq, but does not employ cross linking methods and utilizes microarray analysis instead of sequencing.
  • SELEX: Employed to determine consensus binding sequences.
  • Competition-ChIP: Measures relative replacement dynamics on DNA.
  • ChiRP-Seq: Measures RNA-bound DNA and proteins.
  • ChIP-exo: Employs exonuclease treatment to achieve up to single base-pair resolution
  • ChIP-nexus: Potential improvement on ChIP-exo, capable of achieving up to single base-pair resolution.
  • DRIP-seq: Employs S9.6 antibody to precipitate three-stranded DND:RNA hybrids called R-loops.
  • TCP-seq: Principally similar method to measure mRNA translation dynamics.
  • DamID: Uses enrichment of methylated DNA sequences to detect protein-DNA interaction without antibodies.
  • CUT&RUN: Uses protein A-Mnase

See also

References

  1. "CUT&Tag: a higher resolution, lower cost way to map chromatin". Fred Hutchinson Cancer Research Center. 29 April 2019. Retrieved 23 December 2019.
  2. ^ Kaya-Okur HS, Wu SJ, Codomo ES, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S (April 2019). "CUT&Tag for efficient epigenomic profiling of small samples and single cells". Nature Communications. 10 (1): 1930. Bibcode:2019NatCo..10.1930K. doi:10.1038/s41467-019-09982-5. PMC 6488672. PMID 31036827.
  3. Meyer CA, Liu XS (November 2014). "Identifying and mitigating bias in next-generation sequencing methods for chromatin biology". Nature Reviews. Genetics. 15 (11): 709–21. doi:10.1038/nrg3788. PMC 4473780. PMID 25223782.
  4. Baranello L, Kouzine F, Sanford S, Levens D (May 2016). "ChIP bias as a function of cross-linking time". Chromosome Research. 24 (2): 175–81. doi:10.1007/s10577-015-9509-1. PMC 4860130. PMID 26685864.
  5. He C, Bonasio R (February 2017). "A cut above". eLife. 6. doi:10.7554/eLife.25000. PMC 5310838. PMID 28199181.
  6. Skene PJ, Henikoff S (January 2017). "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites". eLife. 6. doi:10.7554/eLife.21856. PMC 5310842. PMID 28079019.
  7. "Still Using ChIP? Try CUT&RUN for Enhanced Chromatin Profiling". EpiCypher. Retrieved 2019-07-26.
  8. Kaya-Okur, Hatice; Henikoff, Steven. "Bench top CUT&Tag: for efficient epigenomic profiling of small samples and single cells v2 (protocols.io.zcpf2vn)". protocols.io. doi:10.17504/protocols.io.zcpf2vn. S2CID 214175980. Archived from the original on 2013-08-19. Retrieved 2020-07-11.
  9. Kaya-Okur, Hatice; Henikoff, Steven. "Bench top CUT&Tag: for efficient epigenomic profiling of small samples and single cells v1 (protocols.io.zcpf2vn)". protocols.io. doi:10.17504/protocols.io.wnufdew. Archived from the original on 2013-08-19. Retrieved 2020-07-11.
  10. Lan, James H.; Yin, Yuxin; Reed, Elaine F.; Moua, Kevin; Thomas, Kimberly; Zhang, Qiuheng (March 2015). "Impact of three Illumina library construction methods on GC bias and HLA genotype calling". Human Immunology. 76 (2–3): 166–175. doi:10.1016/j.humimm.2014.12.016. PMC 5089167. PMID 25543015.
  11. Sato, Mitsuhiko P; Ogura, Yoshitoshi; Nakamura, Keiji; Nishida, Ruriko; Gotoh, Yasuhiro; Hayashi, Masahiro; Hisatsune, Junzo; Sugai, Motoyuki; Takehiko, Itoh; Hayashi, Tetsuya (1 October 2019). "Comparison of the sequencing bias of currently available library preparation kits for Illumina sequencing of bacterial genomes and metagenomes". DNA Research. 26 (5): 391–398. doi:10.1093/dnares/dsz017. PMC 6796507. PMID 31364694.
  12. Chen, Yen-Chun; Liu, Tsunglin; Yu, Chun-Hui; Chiang, Tzen-Yuh; Hwang, Chi-Chuan (29 April 2013). "Effects of GC Bias in Next-Generation-Sequencing Data on De Novo Genome Assembly". PLOS ONE. 8 (4): e62856. Bibcode:2013PLoSO...862856C. doi:10.1371/journal.pone.0062856. PMC 3639258. PMID 23638157.
Category: