| Calf-intestinal alkaline phosphatase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | Bos taurus | ||||||
| Symbol | ALPI | ||||||
| UniProt | P19111 | ||||||
| |||||||
| Calf-intestinal alkaline phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA. This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.
Applications
Calf-intestinal alkaline phosphatase can serve as an effective tool for removing uranium from groundwater and soil that can pose major health risks. Furthermore, the toxicity of lipopolysaccharide (LPS) was mitigated by calf-intestinal alkaline phosphatase in mice and piglets, which indicates that it could be a promising new therapeutic agent for treating diseases associated with LPS.
References
- Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
- Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM (December 1977). "Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone". Nature. 270 (5637): 486–494. Bibcode:1977Natur.270..486S. doi:10.1038/270486a0. PMID 339105. S2CID 4196683.
- Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM (June 1977). "Rat insulin genes: construction of plasmids containing the coding sequences". Science. 196 (4296). New York, N.Y.: 1313–1319. Bibcode:1977Sci...196.1313U. doi:10.1126/science.325648. PMID 325648.
- Chaudhuri G, Dey P, Dalal D, Venu-Babu P, Thilagaraj WR (July 2013). "A Novel Approach to Precipitation of Heavy Metals from Industrial Effluents and Single-Ion Solutions Using Bacterial Alkaline Phosphatase". Water, Air, & Soil Pollution. 224 (7): 1625. Bibcode:2013WASP..224.1625C. doi:10.1007/s11270-013-1625-y. ISSN 0049-6979. S2CID 97367168.
- Beumer C, Wulferink M, Raaben W, Fiechter D, Brands R, Seinen W (November 2003). "Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets". The Journal of Pharmacology and Experimental Therapeutics. 307 (2): 737–744. doi:10.1124/jpet.103.056606. PMID 12970380. S2CID 15049304.