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Czapek medium, also called Czapek's agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It was named after its inventors, Czech botanist Friedrich Johann Franz Czapek (May 16, 1868 – July 31, 1921) and American chemist Arthur Wayland Dox (September 19, 1882 – 1954). It was developed to grow Aspergillus niger and Penicillium camemberti. It works well for many saprophytic fungi and soil bacteria such as species of Aspergillus, Candida, Penicillium, and Paecilomyces.

Friedrich Czapek's original recipe is as follows:

• 1000 g distilled water
• 30 g cane sugar – energy source and sole source of carbon
• 1 g dipotassium phosphate – buffering agent
• 0.5 g magnesium sulfate – source of cations
• 0.5 g potassium chloride – source of essential ions
• 0.01 g iron sulfate – source of cations

Arthur Wayland Dox added 2 g of sodium nitrate in his version, to provide a sole source of nitrogen that is inorganic. This makes the medium a selective growth medium as only organisms that can use inorganic nitrogen can grow. Czapek and Dox did not add agar but many recipes add 15 g to make a solid medium.

References

1. "Czapek Agar (CZA) Recipe". theLabRat.com. 2005. Archived from the original on 16 March 2015. Retrieved 7 November 2017. Czapek Agar (CZA) Recipe
2. "NRRL Medium No. 8 Czapek's Solution Agar ( CZA )" (PDF). ARS (NRRL) Culture Collection. Peoria, IL: ARS Culture Collection National Center for Agricultural Utilization Research. Archived (PDF) from the original on 27 December 2016. Retrieved 7 November 2017. Czapek's Solution Agar ( CZA )
3. ^ Czapek, F (1901–1902). "Untersuchungen über die Stickstoffgewinnung und Eiweifsbildung der Pflanzen" [Studies on nitrogen production and protein formation of plants] (PDF). Beiträge zur chemischen Physiologie und Pathologie (in German). 1 (12): 538–560. OCLC 1519369. Archived from the original on 28 March 2018. Retrieved 2 October 2017.
4. ^ Dox, Arthur Wayland (1910). "The intracellular enzyms of penicillium and aspergillus: with special reference to those of Penicillium camemberti" (PDF). Bulletin (United States Bureau of Animal Industry). 120: 37. OCLC 22281943. Archived from the original on 6 October 2022. Retrieved 3 October 2017.
5. ^ "CZAPEK MEDIUM" (PDF). Thermo Fisher Scientific. Lenexa, KS: Remel. September 20, 2007. Archived (PDF) from the original on 17 November 2017. Retrieved 3 October 2017.
6. ^ "CZAPEK-DOX AGAR". Hardy Diagnostics Instructions for Use. Santa Maria, CA: Hardy Diagnostics. Archived from the original on 8 July 2017. Retrieved 3 October 2017.

External links

• Czapek Medium recipe
• Czapek solution agar recipe
• Czapek's Agar recipe
• Czapek-Dox Agar Archived 2017-07-08 at the Wayback Machine

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