DNO may refer to:
- Danish Nurses' Organization, a trade union for nurses in Denmark
- Director of Naval Ordnance, British Admiralty administration
- Distribution network operator, companies licensed to distribute electricity in Great Britain
- Dno (air base), a former air base in Russia located 4 km south of Dno
- DNO ASA, a Norwegian oil company
- Drang nach Osten, a term coined in the 19th century to designate German expansion into Slavic lands
- Dutch National Opera, based in Amsterdam, the Netherlands
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