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Formula | C22H27FN4O2 |
Molar mass | 398.482 g·mol |
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DPA-714 or N,N-diethyl-2--5,7-dimethylpyrazolopyrimidine-3-acetamide is a selective ligand for the translocator protein (TSPO) currently under evaluation for several clinical applications. For this reason, a practical, multigram synthetic route for its preparation has been described.
The binding affinity of DPA-714 for TSPO is reported as Ki = 7.0 ± 0.4 nM.
DPA-714 is currently under investigation as a potential radiopharmaceutical for imaging TSPO in living systems using positron emission tomography (PET). DPA-714, along with other members of the DPA class of TSPO ligands, has been shown to decrease microglial activation and increase neuronal survival in a quinolinic acid rat model of excitotoxic neurodegeneration, suggesting potential neuroprotective effects.
See also
References
- Reynolds A, Hanani R, Hibbs D, Damont A, Da Pozzo E, Selleri S, et al. (October 2010). "Pyrazolopyrimidine acetamides: 4-Phenyl alkyl ether derivatives as potent ligands for the 18 kDa translocator protein (TSPO)". Bioorganic & Medicinal Chemistry Letters. 20 (19): 5799–5802. doi:10.1016/j.bmcl.2010.07.135. PMID 20727749.
- Banister SD, Wilkinson SM, Hanani R, Reynolds AJ, Hibbs DE, Kassiou M (2012). "A practical, multigram synthesis of the 2-(2-(4-alkoxyphenyl)-5,7-dimethylpyrazolopyrimidin-3-yl)acetamide (DPA) class of high affinity translocator protein (TSPO) ligands". Tetrahedron Letters. 53 (29): 3780–3783. doi:10.1016/j.tetlet.2012.05.044.
- James ML, Fulton RR, Vercoullie J, Henderson DJ, Garreau L, Chalon S, et al. (May 2008). "DPA-714, a new translocator protein-specific ligand: synthesis, radiofluorination, and pharmacologic characterization". Journal of Nuclear Medicine. 49 (5): 814–822. doi:10.2967/jnumed.107.046151. PMID 18413395.
- Damont A, Hinnen F, Kuhnast B, Schöllhorn-Peyronneau MA, James M, Luus C, Tavitian B, Kassiou M, Dollé F (2008). "Radiosynthesis of DPA-714, a selective radioligand for imaging the translocator protein (18 kDa) with PET". Journal of Labelled Compounds and Radiopharmaceuticals. 51 (7): 286–292. doi:10.1002/jlcr.1523.
- Leaver KR, Reynolds A, Bodard S, Guilloteau D, Chalon S, Kassiou M (February 2012). "Effects of translocator protein (18 kDa) ligands on microglial activation and neuronal death in the quinolinic-acid-injected rat striatum". ACS Chemical Neuroscience. 3 (2): 114–119. doi:10.1021/cn200099e. PMC 3369788. PMID 22860181.
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