Exo-(1-4)-α-D-glucan lyase

The enzyme exo-(1→4)-α-D-glucan lyase (EC 4.2.2.13, α-(1→4)-glucan 1,5-anhydro-D-fructose eliminase, α-1,4-glucan exo-lyase, α-1,4-glucan lyase, GLase) is an enzyme with systematic name (1→4)-α-D-glucan exo-4-lyase (1,5-anhydro-D-fructose-forming). This enzyme catalyses the following chemical reaction

linear α-glucan = (n-1) 1,5-anhydro-D-fructose + D-glucose

The enzyme catalyses the sequential degradation of (1→4)-α-D-glucans from the non-reducing end.

References

Yu S, Kenne L, Pedersén M (March 1993). "Alpha-1,4-glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds". Biochimica et Biophysica Acta (BBA) - General Subjects. 1156 (3): 313–20. doi:10.1016/0304-4165(93)90049-e. PMID 8461323.
Yu S, Pedersén M (1993). "Alpha-1,4-glucan lyase, a new class of starch/glycogen-degrading enzyme. II. Subcellular localization and partial amino-acid sequence". Planta. 191 (1): 137–42. doi:10.1007/bf00240905. PMID 7763826. S2CID 39773299.
Yu S, Ahmad T, Kenne L, Pedersén M (May 1995). "alpha-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. III. Substrate specificity, mode of action, and cleavage mechanism". Biochimica et Biophysica Acta (BBA) - General Subjects. 1244 (1): 1–9. doi:10.1016/0304-4165(94)00202-9. PMID 7766642.
Yu S, Christensen TM, Kragh KM, Bojsen K, Marcussen J (May 1997). "Efficient purification, characterization and partial amino acid sequencing of two alpha-1,4-glucan lyases from fungi". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1339 (2): 311–20. doi:10.1016/s0167-4838(97)00014-9. PMID 9187252.
Yu S, Bojsen K, Svensson B, Marcussen J (August 1999). "alpha-1,4-glucan lyases producing 1,5-anhydro-D-fructose from starch and glycogen have sequence similarity to alpha-glucosidases". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1433 (1–2): 1–15. doi:10.1016/s0167-4838(99)00152-1. PMID 10446355.
Lee SS, Yu S, Withers SG (May 2002). "alpha-1,4-Glucan lyase performs a trans-elimination via a nucleophilic displacement followed by a syn-elimination". Journal of the American Chemical Society. 124 (18): 4948–9. doi:10.1021/ja0255610. PMID 11982345.
Lee SS, Yu S, Withers SG (November 2003). "Detailed dissection of a new mechanism for glycoside cleavage: alpha-1,4-glucan lyase". Biochemistry. 42 (44): 13081–90. doi:10.1021/bi035189g. PMID 14596624.

External links

Exo-(1->4)-alpha-D-glucan+lyase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Carbon–oxygen lyases (EC 4.2) (primarily dehydratases)
4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase
4.2.2: Acting on polysaccharides	Hyaluronate lyase Pectin lyase
4.2.3: Acting on phosphates	Threonine synthase
4.2.99: Other	Carboxymethyloxysuccinate lyase Hydroperoxide lyase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 4.2.2