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FAM83H
Identifiers
Aliases	FAM83H, AI3, family with sequence similarity 83 member H, AI3A
External IDs	OMIM: 611927; MGI: 2145900; HomoloGene: 15890; GeneCards: FAM83H; OMA:FAM83H - orthologs
Gene location (Human) Chr. Chromosome 8 (human) Band 8q24.3 Start 143,723,933 bp End 143,738,234 bp
Gene location (Mouse) Chr. Chromosome 15 (mouse) Band 15|15 D3 Start 75,872,942 bp End 75,886,185 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right uterine tube skin of leg skin of abdomen mucosa of transverse colon olfactory zone of nasal mucosa right lobe of thyroid gland left lobe of thyroid gland minor salivary glands body of pancreas right lobe of liver Top expressed in lip corneal stroma lumbar spinal ganglion conjunctival fornix hair follicle left colon transitional epithelium of urinary bladder esophagus skin of external ear ileum More reference expression data BioGPS n/a
Gene ontology Molecular function protein kinase binding keratin filament binding Cellular component cytoplasm keratin filament cytoskeleton Biological process biomineral tissue development protein localization to cytoskeleton intermediate filament cytoskeleton organization positive regulation of cell migration Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 286077 105732 Ensembl ENSG00000180921 ENSG00000273889 ENSMUSG00000046761 UniProt Q6ZRV2 Q148V8 RefSeq (mRNA) NM_198488 NM_001168253 NM_134087 RefSeq (protein) NP_940890 NP_001161725 NP_598848 Location (UCSC) Chr 8: 143.72 – 143.74 Mb Chr 15: 75.87 – 75.89 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

FAM83H is a protein, which in humans is encoded by the FAM83H gene. The protein is also known as uncharacterized protein FAM83H. FAM83H is targeted for the nucleus. It is predicted to play a role in the structural development and calcification of tooth enamel.

Gene

Location

FAM83H is located on the long arm of chromosome 8 (8q24.3), starting at 143723933 and ending at 143738030. The FAM83H gene spans 14097 base pairs and is orientated on the—strand. The coding region is made up of 5,604 base pairs and 5 exons.

Expression

FAM83H is ubiquitously expressed throughout the human body at relatively low levels.

Transcript Variants

In humans, there is only one known major product of the FAM83H gene.

Homology

Paralogs

There are no paralogs of FAM83H.

Orthologs

Below is a table of orthologs to the human FAM83H protein. The table include closely, intermediately and distantly related orthologs.

Orthologs of the human protein FAM83H are listed above in descending order or date of divergence and then ascending order of percent identity. FAM83H is highly conserved throughout all orthologs, demonstrated by a 40% identity in the least similar ortholog. FAM83H has evolved slowly and evenly over time.

Protein

General Properties

The molecular weight of FAM83H is 127.1kD and contains 1179 amino acids. The isoelectric point is 6.52. There are no significant positive or negative charge clusters in the protein. There is a stretch of 21 0’s from 254-275 and a stretch of 24 0’s from 420-444.1

Composition

FAM83H is proline rich, being 10.32% protein, and is asparagine deficient with only 1.1%. The percent composition of each amino acid is fairly consistent throughout the orthologs of the protein. The most distant ortholog displays the most variance in amino acid composition.

Domains

FAM83H has two known domains. The PLDc_FAM83H (phospholipase like domain) domain stretches from 17-281 on FAM83H. It lacks the functionally important histidine, so while it may share similar structure it most likely lacks PLD activity. The MIP-T3 microtubule binding domain stretches from 909-1176.

Post-translational modifications

FAM83H is highly phosphorylated post modification. There are 11 predicted phosphorylated sites. There are two motifs with high probability of post translational modification sumoylation sites. Sumoylation sites are involved in a number of cellular processes, including nuclear-cytosolic transport, transcriptional regulation and protein stability. FAM83H does not have a signal peptide

Secondary Structure

Fam83H is primarily composed of alpha helices and random coils. Alpha helices comprise the majority of the protein. There is a transmembrane domain from 231-252.

Subcellular Localization

Protein FAM83H is targeted to the nucleus.

Interacting Proteins

FAM83H was found to interact with WDR72 and MMP20. MMP20 is responsible for the breakdown of extracellular matrix and plays a role in tissue remodeling in ameloblasts. mutations in WDR72 is thought to play a role in amelogenesis imperfecta

Clinical Significance

Disease Association

People who suffer from amelogenesis imperfecta have lost function in FAM83H.

References

1. ^ ENSG00000273889 GRCh38: Ensembl release 89: ENSG00000180921, ENSG00000273889 – Ensembl, May 2017
2. ^ GRCm38: Ensembl release 89: ENSMUSG00000046761 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "NCBI gene database". NCBI.
6. "GEO profiles". NCBI geo profiles.
7. "EST profiles". NCBI EST profiles.
8. "Emsembl". Vega.
9. "Genecards". The Gene Human Database.
10. "Aceview". NCBI.
11. "Genecards". The Gene Human Database.
12. "BLAST". NCBI.
13. Hedges SB. "TimeTree". Bioinformatics.
14. "SAPS". Statistical Analysis of Protein Sequence, Biology Workbench.
15. "NCBI Structure". The Gene Human Database.
16. "PELE". San Diego Supercomputer Center.
17. "CHOFAS (Predict Secondary Structure of PS". Chou-Fasman. Archived from the original on 2003-08-11. Retrieved 2015-05-09.
18. "PSORT II". Expasy.
19. "IntAct". EMNL-EBI.
20. "NCBI gene database". NCBI.
21. "Genecards". The Gene Human Database.

Further reading

• Kim JW, Lee SK, Lee ZH, et al. (2008). "FAM83H mutations in families with autosomal-dominant hypocalcified amelogenesis imperfecta". Am. J. Hum. Genet. 82 (2): 489–94. doi:10.1016/j.ajhg.2007.09.020. PMC 2427219. PMID 18252228.
• Ding Y, Estrella MR, Hu YY, et al. (2009). "Fam83h is associated with intracellular vesicles and ADHCAI". J. Dent. Res. 88 (11): 991–6. doi:10.1177/0022034509349454. PMC 2835506. PMID 19828885.
• Hart PS, Becerik S, Cogulu D, et al. (2009). "Novel FAM83H mutations in Turkish families with autosomal dominant hypocalcified amelogenesis imperfecta". Clin. Genet. 75 (4): 401–4. doi:10.1111/j.1399-0004.2008.01112.x. PMC 4264522. PMID 19220331.
• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Lee SK, Hu JC, Bartlett JD, et al. (2008). "Mutational spectrum of FAM83H: the C-terminal portion is required for tooth enamel calcification". Hum. Mutat. 29 (8): E95–9. doi:10.1002/humu.20789. PMC 2889227. PMID 18484629.
• El-Sayed W, Shore RC, Parry DA, et al. (2010). "Ultrastructural analyses of deciduous teeth affected by hypocalcified amelogenesis imperfecta from a family with a novel Y458X FAM83H nonsense mutation". Cells Tissues Organs (Print). 191 (3): 235–9. doi:10.1159/000252801. PMC 4432877. PMID 20160442.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Brandenberger R, Wei H, Zhang S, et al. (2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197. S2CID 27764390.
• Wright JT, Frazier-Bowers S, Simmons D, et al. (2009). "Phenotypic variation in FAM83H-associated amelogenesis imperfecta". J. Dent. Res. 88 (4): 356–60. doi:10.1177/0022034509333822. PMC 2754853. PMID 19407157.
• Hyun HK, Lee SK, Lee KE, et al. (2009). "Identification of a novel FAM83H mutation and microhardness of an affected molar in autosomal dominant hypocalcified amelogenesis imperfecta". International Endodontic Journal. 42 (11): 1039–43. doi:10.1111/j.1365-2591.2009.01617.x. PMID 19825039.

• Genes on human chromosome 8