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FGF21
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5VAQ, 6M6F, 6M6E
Identifiers
Aliases	FGF21, fibroblast growth factor 21
External IDs	OMIM: 609436; MGI: 1861377; HomoloGene: 10428; GeneCards: FGF21; OMA:FGF21 - orthologs
Gene location (Human) Chr. Chromosome 19 (human) Band 19q13.33 Start 48,755,524 bp End 48,758,333 bp
Gene location (Mouse) Chr. Chromosome 7 (mouse) Band 7|7 B3 Start 45,263,310 bp End 45,264,914 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right lobe of liver pancreatic ductal cell muscle of thigh breast mammary gland lactiferous gland gastrocnemius muscle head mouth blood Top expressed in pyloric antrum embryo thymus calvaria liver islet of Langerhans left lobe of liver axial skeleton cornea spermatid More reference expression data BioGPS More reference expression data
Gene ontology Molecular function growth factor activity protein binding fibroblast growth factor receptor binding Cellular component extracellular region extracellular space Biological process endothelial cell apoptotic process positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway positive regulation of ERK1 and ERK2 cascade positive regulation of cell population proliferation positive regulation of glucose import cell-cell signaling cellular response to glucose stimulus fibroblast growth factor receptor signaling pathway regulation of low-density lipoprotein particle clearance response to nutrient levels negative regulation of endothelial cell apoptotic process response to activity signal transduction cellular response to low-density lipoprotein particle stimulus endoplasmic reticulum unfolded protein response negative regulation of neuron death positive regulation of triglyceride catabolic process response to methionine cellular response to glucagon stimulus regulation of signaling receptor activity positive regulation of cold-induced thermogenesis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 26291 56636 Ensembl ENSG00000105550 ENSMUSG00000030827 UniProt Q9NSA1 Q9JJN1 RefSeq (mRNA) NM_019113 NM_020013 RefSeq (protein) NP_061986 NP_064397 Location (UCSC) Chr 19: 48.76 – 48.76 Mb Chr 7: 45.26 – 45.26 Mb PubMed search
Wikidata
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Fibroblast growth factor 21 (FGF-21) is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family and specifically a member of the endocrine subfamily which includes FGF23 and FGF15/19. FGF21 is the primary endogenous agonist of the FGF21 receptor, which is composed of the co-receptors FGF receptor 1 and β-Klotho.

FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGFs act through a family of four FGF receptors. Binding is complicated and requires both interaction of the FGF molecule with an FGF receptor and binding to heparin through a heparin binding domain. Endocrine FGFs lack a heparin binding domain and thus can be released into the circulation.

FGF21 is a hepatokine – i.e., a hormone secreted by the liver – that regulates simple sugar intake and preferences for sweet foods via signaling through FGF21 receptors in the paraventricular nucleus of the hypothalamus and correlates with reduced dopamine neurotransmission within the nucleus accumbens.

A single-nucleotide polymorphism of the FGF21 gene – the FGF21 rs838133 variant (frequency 44.7%) – has been identified as a genetic mechanism responsible for the sweet tooth behavioral phenotype, a trait associated with cravings for sweets and high sugar consumption, in both humans and mice.

Regulation

FGF21 is specifically induced by mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) activity. The oxidized form of ketone bodies (acetoacetate) in a cultured medium also induced FGF21, possibly via a sirtuin 1 (SIRT1)-dependent mechanism. HMGCS2 activity has also been shown to be increased by deacetylation of lysines 310, 447, and 473 via SIRT3 in the mitochondria.

While FGF21 is expressed in numerous tissues, including liver, brown adipose tissue, white adipose tissue (WAT) and pancreas, circulating levels of FGF21 are derived specifically from the liver in mice. In liver FGF21 expression is regulated by PPARα and levels rise substantially with both fasting and consumption of ketogenic diets.

Liver X receptor (LXR) represses FGF21 in humans via an LXR response element located from -37 to -22 bp on the human FGF21 promoter.

Function

FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Treatment of animals with FGF21 results in increased energy expenditure, fat utilization and lipid excretion.

β-Klotho (KLB) functions as a cofactor essential for FGF21 activity.

In cows plasma FGF21 was nearly undetectable in late pregnancy (LP), peaked at parturition, and then stabilized at lower, chronically elevated concentrations during early lactation (EL). Plasma FGF21 was similarly increased in the absence of parturition when an energy-deficit state was induced by feed restricting late-lactating dairy cows, implicating energy insufficiency as a cause of chronically elevated FGF21 in EL. The liver was the major source of plasma FGF21 in early lactation with little or no contribution by WAT, skeletal muscle, and mammary gland. Meaningful expression of the FGF21 coreceptor β-Klotho was restricted to liver and WAT in a survey of 15 tissues that included the mammary gland. Expression of β-Klotho and its subset of interacting FGF receptors was modestly affected by the transition from LP to EL in liver but not in WAT.

Clinical significance

Serum FGF-21 levels were significantly increased in patients with type 2 diabetes mellitus (T2DM) which may indicate a role in the pathogenesis of T2DM. Elevated levels also correlate with liver fat content in non-alcoholic fatty liver disease and positively correlate with BMI in humans suggesting obesity as a FGF21-resistant state.

A single-nucleotide polymorphism (SNP) of the FGF21 gene – the FGF21 rs838133 variant (frequency 44.7%) – has been identified as a genetic mechanism responsible for the sweet tooth behavioral phenotype, a trait associated with cravings for sweets and high sugar consumption, in both humans and mice.

Animal studies

Mice lacking FGF21 fail to fully induce PGC-1α expression in response to a prolonged fast and have impaired gluconeogenesis and ketogenesis.

FGF21 stimulates phosphorylation of fibroblast growth factor receptor substrate 2 and ERK1/2 in the liver. Acute FGF21 treatment induced hepatic expression of key regulators of gluconeogenesis, lipid metabolism, and ketogenesis including glucose-6-phosphatase, phosphoenol pyruvate carboxykinase, 3-hydroxybutyrate dehydrogenase type 1, and carnitine palmitoyltransferase 1α. In addition, injection of FGF21 was associated with decreased circulating insulin and free fatty acid levels. FGF21 treatment induced mRNA and protein expression of PGC-1α, but in mice PGC-1α expression was not necessary for the effect of FGF21 on glucose metabolism.

In mice FGF21 is strongly induced in liver by prolonged fasting via PPAR-alpha and in turn induces the transcriptional coactivator PGC-1α and stimulates hepatic gluconeogenesis, fatty acid oxidation, and ketogenesis. FGF21 also blocks somatic growth and sensitizes mice to a hibernation-like state of torpor, playing a key role in eliciting and coordinating the adaptive starvation response. FGF21 expression is also induced in white adipose tissue by PPAR-gamma, which may indicate it also regulates metabolism in the fed state. FGF21 is induced in both rodents and humans consuming a low protein diet. FGF21 expression is also induced by diets with reduced levels of the essential dietary amino acids methionine or threonine, or with reduced levels of branched-chain amino acids.

Activation of AMPK and SIRT1 by FGF21 in adipocytes enhanced mitochondrial oxidative capacity as demonstrated by increases in oxygen consumption, citrate synthase activity, and induction of key metabolic genes. The effects of FGF21 on mitochondrial function require serine/threonine kinase 11 (STK11/LKB1), which activates AMPK. Inhibition of AMPK, SIRT1, and PGC-1α activities attenuated the effects of FGF21 on oxygen consumption and gene expression, indicating that FGF21 regulates mitochondrial activity and enhances oxidative capacity through an LKB1-AMPK-SIRT1-PGC-1α-dependent mechanism in adipocytes, resulting in increased phosphorylation of AMPK, increased cellular NAD+ levels and activation of SIRT1 and deacetylation of SIRT1 targets PGC-1α and histone 3.

Acutely, the rise in FGF21 in response to alcohol consumption inhibits further drinking. Chronically, the rise in FGF21 expression in the liver may protect against liver damage.

References

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Further reading

• Chen WW, Li L, Yang GY, Li K, Qi XY, Zhu W, Tang Y, Liu H, Boden G (January 2008). "Circulating FGF-21 levels in normal subjects and in newly diagnose patients with Type 2 diabetes mellitus". Experimental and Clinical Endocrinology & Diabetes. 116 (1): 65–8. doi:10.1055/s-2007-985148. PMID 17926232. S2CID 260134526.
• Kharitonenkov A, Wroblewski VJ, Koester A, Chen YF, Clutinger CK, Tigno XT, Hansen BC, Shanafelt AB, Etgen GJ (February 2007). "The metabolic state of diabetic monkeys is regulated by fibroblast growth factor-21". Endocrinology. 148 (2): 774–81. doi:10.1210/en.2006-1168. PMID 17068132.
• Zhang Z, Henzel WJ (October 2004). "Signal peptide prediction based on analysis of experimentally verified cleavage sites". Protein Science. 13 (10): 2819–24. doi:10.1110/ps.04682504. PMC 2286551. PMID 15340161.
• Popovici C, Conchonaud F, Birnbaum D, Roubin R (September 2004). "Functional phylogeny relates LET-756 to fibroblast growth factor 9". The Journal of Biological Chemistry. 279 (38): 40146–52. doi:10.1074/jbc.M405795200. PMID 15199049.
• Youm YH, Horvath TL, Mangelsdorf DJ, Kliewer SA, Dixit VD (January 2016). "Prolongevity hormone FGF21 protects against immune senescence by delaying age-related thymic involution". Proceedings of the National Academy of Sciences of the United States of America. 113 (4): 1026–31. Bibcode:2016PNAS..113.1026Y. doi:10.1073/pnas.1514511113. PMC 4743827. PMID 26755598.

External links

• fibroblast+growth+factor+21 at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• "FGF21". Information Hyperlinked over Proteins. ihop-net.org. Retrieved August 12, 2008.
• "FGF21". Gene Cards. Weizmann Institute of Science. Retrieved August 12, 2008.
• Got a sweet tooth? Blame your liver Phys.org, 2017

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

• Genes on human chromosome 19
• Aging-related proteins
• Anti-aging substances