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Ficolins are pattern recognition receptors that bind to acetyl groups present in the carbohydrates of bacterial surfaces and mediate activation of the lectin pathway of the complement cascade.

Structure

Ficolins (Fi+Col+Lin) are a group of oligomeric lectins with N-terminal collagen-like domain and a C-terminal fibrinogen-like domain. The primary ficolin structure contains 288 amino acids. The combination of collagen-like and fibrinogen-like domain allows the protein to form a basic subunit containing a triple helical tail and a trio of globular heads.

Ficolins are produced in the liver by hepatocytes and in the lung by alveolar cells type II, neutrophils and monocytes.

Role in innate immunity

We now know that innate immune recognition mechanisms are sophisticated. Exocrine secretions provide a variety of soluble factors that are able to protect the body from potential pathogens.

Together with pentraxins, collectins and C1q molecules, ficolins constitute the soluble pattern-recognition molecules (PRMs) which play an important role in humoral innate immunity. Ficolins recognise carbohydrate structures on pathogens' surfaces as their pathogen-associated molecular pattern (PAMP) and activate the lectin pathway of the complement cascade. Specifically, ficolins bind to acetyl groups present in certain bacterial molecules, such as N-acetylglucosamine, a component of peptidoglycan in the bacterial cell wall. When ficolins bind to their PAMP ligands by their C-terminal fibrinogen-like domain, they initiate the proteolytic complement cascade, facilitated by the mannose-binding protein-associated serine proteases (MASPs) that ficolins are associated to and co-circulate with. Serine proteases then cleave a number of soluble complement proteins leading to complement activation, opsonisation, generation of proinflammatory mediators, and cell lysis.

Collectins and ficolins are also called collagenous lectins. The collectin family constitutes calcium-dependent proteins. In contrast, the ficolin family does not bind to PAMPs in a calcium-dependent way.

Types of ficolin

Three ficolins have been identified in humans:

1. M-ficolin (FCN1), monocyte ficolin
2. L-ficolin (FCN2), liver ficolin
3. H-ficolin (FCN3), hakata antigen.

Ficolin-1 and ficolin-2 are encoded be a gene localised on chromosome 9 (9q34) and they share approximately 80% identity in amino sequence. Whereas, ficolin-3 is encoded by chromosome 1 and therefore it has only about 50% identity with the other two ficolins. A cross-reactivity of the ficolins in human serum has been observed.

Clinical references

The concentration of ficolins in healthy serum is between 3 and 5 μg/mL.

As Ficolin-2 and 3 are expressed by hepatocytes, their levels decrease in advanced liver diseases like cirrhosis. Low ficolin levels contribute to cirrhosis-associated immune dysfunction.

Immunologist Jeak L. Ding and her team found that natural IgG (nIgG; a non-specific immunoglobulin of adaptive immunity) is not quiescent, but plays a crucial role in immediate immune defense by collaborating with ficolin (an innate immune protein).

References

1. ^ Merle, Nicolas S.; Church, Sarah Elizabeth; Fremeaux-Bacchi, Veronique; Roumenina, Lubka T. (2015). "Complement System Part I - Molecular Mechanisms of Activation and Regulation". Frontiers in Immunology. 6: 262. doi:10.3389/fimmu.2015.00262. PMC 4451739. PMID 26082779. 262.
2. ^ Kilpatrick, David C.; Chalmers, James D. (2012). "Human L-Ficolin (Ficolin-2) and Its Clinical Significance". Journal of Biomedicine and Biotechnology. 2012: 138797. doi:10.1155/2012/138797. ISSN 1110-7243. PMC 3303570. PMID 22500076.
3. ^ Matsushita, Misao (2018). "Chapter 5 - Ficolins". In Barnum, Scott R.; Schein, Theresa N. (eds.). The Complement FactsBook (Second ed.). Elsevier. pp. 45–56. doi:10.1016/B978-0-12-810420-0.00005-5. ISBN 978-0-12-810420-0.
4. ^ Hajishengallis, George; Russell, Michael W. (2015). "Chapter 15 - Innate Humoral Defense Factors". In Mestecky, Jiri; Strober, Warren; Russell, Michael W.; Kelsall, Brian L.; Cheroutre, Hilde; Lambrecht, Bart N. (eds.). Mucosal Immunology (Fourth ed.). Elsevier. pp. 251–270. doi:10.1016/b978-0-12-415847-4.00015-x. ISBN 978-0-12-415847-4.
5. Endo, Yuichi; Matsushita, Misao; Fujita, Teizo (June 2007). "Role of ficolin in innate immunity and its molecular basis". Immunobiology. 212 (4–5): 371–379. doi:10.1016/j.imbio.2006.11.014. ISSN 0171-2985. PMID 17544822.
6. ^ Krarup, Anders; Thiel, Steffen; Hansen, Annette; Fujita, Teizo; Jensenius, Jens C. (2004). "L-ficolin Is a Pattern Recognition Molecule Specific for Acetyl Groups". Journal of Biological Chemistry. 279 (46): 47513–47519. doi:10.1074/jbc.M407161200. PMID 15331601.
7. ^ Jarlhelt, Ida; Pilely, Katrine; Clausen, Jytte Bryde; Skjoedt, Mikkel-Ole; Bayarri-Olmos, Rafael; Garred, Peter (2020-02-24). "Circulating Ficolin-2 and Ficolin-3 Form Heterocomplexes". The Journal of Immunology. 204 (7): 1919–1928. doi:10.4049/jimmunol.1900694. ISSN 0022-1767. PMID 32094208. S2CID 211477247.
8. Foldi, Ildiko; Tornai, Tamas; Tornai, David; Sipeki, Nora; Vitalis, Zsuzsanna; Tornai, Istvan; Dinya, Tamas; Antal-Szalmas, Peter; Papp, Maria (2017). "Lectin-complement pathway molecules are decreased in patients with cirrhosis and constitute the risk of bacterial infections". Liver International. 37 (7): 1023–1031. doi:10.1111/liv.13368. hdl:2437/234045. ISSN 1478-3231. PMID 28109038. S2CID 4724419.
9. Panda, Saswati; Ding, Jeak L. (2015). "Natural Antibodies Bridge Innate and Adaptive Immunity". The Journal of Immunology. 194 (1): 13–20. doi:10.4049/jimmunol.1400844. PMID 25527792.

• Ficolins
• Blood proteins
• Human proteins
• Lectins