Synthetic equivalents of fluorescein dye used in oligonucleotide synthesis
Non-nucleoside phosphoramidites for 5'-modification of synthetic oligonucleotides Fluorescein amidites , abbreviated as FAM , are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology . FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the complementary nucleic acids or primers for polymerase chain reaction . Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons .
The commercially available 6-FAM version is shown in the figure.
References
US 5583236 , Brush, C. K., "Fluorescein Labelled Phosphoramidites"
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