Gambale is a surname. Notable people with the surname include:
- Mickey Gambale ,(born 1975) South African and Italian prominent figure in the South African financial industry
- Ambra Gambale, South African and Italian artist and jewellery designer
- Diego Gambale, (born 1998), Italian footballer
- Frank Gambale (born 1958), Australian jazz guitarist
- Mary Gambale (born 1988), American tennis player
- Giuseppe Gambale [Wikidata] (born 1964), Italian politician
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