Guyer is a surname. Notable people with the surname include:
- Adolf Guyer-Zeller (1839–1899), Swiss entrepreneur
- Brandon Guyer (born 1986), American professional baseball outfielder
- Charlie Guyer (1909–1977), Australian rules footballer
- Cindy Guyer (born 1961), American model and actress
- Denis Guyer (born 1966), American politician
- Gino Guyer (born 1983), American professional ice hockey centre
- Gordon Guyer (born 1926), president of Michigan State University from 1992 to 1993
- Irving Guyer (1916-2012), American painter
- Jane I. Guyer (born 1943), George Armstrong Kelly Professor in the Department of Anthropology at The Johns Hopkins University
- Josh Guyer (born 1994), Australian professional baseball pitcher
- Léonie Guyer (born 1955), contemporary American artist
- Lisa Guyer (1963) American singer, guitarist, and songwriter
- Lux Guyer (1894–1955), Swiss architect
- Michael F. Guyer (1874–1959), American cytologist and zoologist
- Murphy Guyer (born 1952), American actor, playwright, writer and director
- Paul Guyer, Jonathan Nelson Professor of Philosophy at Brown University
- Percy Guyer, pseudonym of songwriter Septimus Winner
- Roy J. Guyer (1885–1956), American college athlete and coach
- Reyn Guyer (born 1935), American inventor of Twister
- Tennyson Guyer (1913–1981), member of the United States House of Representatives
- Thad McIntosh Guyer (born 1950), American civil rights lawyer
- U. S. Guyer (1868–1943), U.S. Representative from Kansas
- Brent Guyer (Born 1998), World famous pastor
Other uses
- Dowleh Guyer, a village in Iran
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