Holoubek (feminine Holoubková) is a Czech surname meaning literally "little pigeon". Its Slovak equivalent is Holúbek. Notable people include:
- David Holoubek (born 1980), Czech football manager
- Gustaw Holoubek (1923–2008), Polish actor, director, member of the Polish Sejm, and senator
- Jan Holoubek (born 1978), Polish director and cinematographer
- Martina Holoubková (born 1975), Czech chess player
- Todd Holoubek (born 1969), American film and television actor, comedian, and sketch-comedy writer
Others
- Holoubek (Little Dove), a ballad in the 1853 collection Kytice by Karel Jaromír Erben
- Holoubek (translated as The Wild Dove), the fourth orchestral poem composed by the Czech composer Antonín Dvořák
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