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2-hydroxyisoflavanone synthase (EC 1.14.13.136, CYT93C, IFS, isoflavonoid synthase) is an enzyme with systematic name liquiritigenin,NADPH:oxygen oxidoreductase (hydroxylating, aryl migration). This enzyme catalyses the following chemical reactions:

liquiritigenin + O₂ + NADPH + H ${\displaystyle \rightleftharpoons }$ 2,4',7-trihydroxyisoflavanone + H₂O + NADP

and

(2S)-naringenin + O₂ + NADPH + H ${\displaystyle \rightleftharpoons }$ 2,4',5,7-tetrahydroxyisoflavanone + H₂O + NADP

Isoflavonoid synthase requires cytochrome P450.

References

1. Hashim MF, Hakamatsuka T, Ebizuka Y, Sankawa U (October 1990). "Reaction mechanism of oxidative rearrangement of flavanone in isoflavone biosynthesis". FEBS Letters. 271 (1–2): 219–22. doi:10.1016/0014-5793(90)80410-k. PMID 2226805.
2. Sawada Y, Kinoshita K, Akashi T, Aoki T, Ayabe S (September 2002). "Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase". The Plant Journal. 31 (5): 555–64. doi:10.1046/j.1365-313x.2002.01378.x. PMID 12207646.
3. Sawada Y, Ayabe S (May 2005). "Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue". Biochemical and Biophysical Research Communications. 330 (3): 907–13. doi:10.1016/j.bbrc.2005.03.053. PMID 15809082.

External links

• Isoflavonoid+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

• Biology

• EC 1.14.13