Isotta may refer to:
- the Italian form of the name Iseult
- Isotta Nogarola (1418–1466), Italian writer and humanist
- Isotta degli Atti (died 1474), an Italian regent
- Isotta Brembati (died 1586), Italian poet and countess
- Isotta Gervasi (1889–1967), Italian medical doctor
- Isotta Brothers (19th century), Italian hoteliers
- Paolo Isotta (1950–2021), Italian musicologist
- Isotta Fraschini, Italian luxury car manufacturer
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