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Johor Route 33
Major junctions
Northeast end	Sungai Mati
Major intersections	FT 23 Federal Route 23 J137 Jalan Panchor J34 Jalan Bukit Serampang
Southwest end	Bukit Serampang
Location
Country	Malaysia
Primary destinations	Serom, Gerisek, Panchor, Bukit Kepong
Highway system
Highways in Malaysia Expressways Federal State

Jalan Bukit Gambir (Johor state route J33) is a major road in Johor, Malaysia. The roads is also a main route to North–South Expressway Southern Route via Bukit Gambir Interchange.

List of junctions

Km	Exit	Junctions	To	Remarks
		Sungai Mati	North FT 23 Tangkak FT 23 Jementah FT 23 Segamat FT 23 Gunung Ledang North–South Expressway Southern Route AH2 North–South Expressway Southern Route Kuala Lumpur Melaka Johor Bahru Singapore Southwest FT 23 Bandar Maharani (Muar) (Royal Town) FT 5 Melaka	Junctions
		Serom
		Bukit Gambir-NSE	North–South Expressway Southern Route AH2 North–South Expressway Southern Route North Kuala Lumpur Melaka Tangkak South Johor Bahru Yong Peng Pagoh	T-junctions with toll plaza
		Bukit Gambir
		Jalan Panchor	Southeast J137 Jalan Panchor Panchor Pagoh North–South Expressway Southern Route AH2 North–South Expressway Southern Route Kuala Lumpur Melaka Johor Bahru Singapore	T-junctions
		Gerisek
		FELDA Bukit Serampang
		Bukit Serampang	J34 Jalan Bukit Serampang West Sagil Jementah Tangkak Segamat East Bukit Kepong Labis	T-junctions

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