Jolene may refer to:
People
- Jolene (name), a female given name
Music
- Jolene (band), an American alt-country band
- Jolene (album), an album by Dolly Parton
- "Jolene" (song), a 1973 song by Dolly Parton
- "Jolene", 2024 variation by Beyoncé of the Dolly Parton song
- "Jolene", a song by Hey Ocean! on the 2012 album IS
- "Jolene", a song by Ewert and The Two Dragons on the 2011 album Good Man Down
- "Jolene", a song by Bob Dylan on the 2009 album Together Through Life
- "Jolene", a song by Ray LaMontagne on the 2004 album Trouble covered on the 2008 album The Foundation by Zac Brown Band
- "Jolene", a song by Cake on the 1994 album Motorcade of Generosity
- "Jolene", a song by The White Stripes on the 2010 album Under Great White Northern Lights
- "Jolene", a trumpet solo by James F. Burke (Musician)
Other uses
- Jolene (film), a 2008 American drama film directed by Dan Ireland
- "Jolene: A Life" a short story by E. L. Doctorow inspired by the Parton song and source material for the Ireland movie
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