Jooste is an Afrikaans language surname. Notable people include:
- Leon Jooste (born 1969), Namibian politician and businessman
- Markus Jooste (born 1961), South African businessman
- Nick Jooste (born 1997), Australian Rugby Union player
- Pamela Jooste, South African novelist
- Tobi Jooste, South African singer, model, writer and television personality
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