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MC3T3

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MC3T3 is an osteoblast precursor cell line derived from Mus musculus (mouse) calvaria.

A number of derivatives of this strain have been isolated to select for varying degrees of osteogenic potential, and have been widely used as model systems in bone biology. A standard textbook calls its MC3T3-E1 sub-line "one of the most convenient and physiologically relevant systems for study of transcriptional control in calvarial osteoblasts." This is a spontaneously transformed ( immortalized ) cell line. As such, it is a very convenient research tool but caution should be used when extrapolating these results to normal cells, and even more, to normal human cells.

When cultured on tissue culture plastic or hydrogels without cell adhesion motifs MC3T3 cells adopt a polygonal shape without considerable polarization and focal adhesions. Beyond a critical density of cell adhesion motifs MC3T3 cells polarize and form stable focal adhesions. MC3T3 cells show higher proliferation and polarization on stiff substrates.

The MC3T3 cell line is also used for modeling skeletal tissue regeneration because its undifferentiated state is phenotypically similar to that of an osteochondroprogenitor cell. One published research article, using MC3T3-E1 cells as a model for studying cartilage regeneration, describes some of the specific limitations of the cell line for understanding behavior in human cells.

References

  1. Kodama H. A., Amagai Y., Sudo H., Kasai S., Yamamoto S. Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jpn J Oral Biol. 1981. Oct;23(4):899-901.
  2. Bilezikian, John P., Lawrence G. Raisz, and Gideon A. Rodan. Principles of Bone Biology. San Diego: Academic Press, 2002. p.1506
  3. Arriero, María del Mar; Ramis, Joana M.; Perelló, Joan; Monjo, Marta (2012). "Differential Response of MC3T3-E1 and Human Mesenchymal Stem Cells to Inositol Hexakisphosphate". Cellular Physiology and Biochemistry. 30 (4): 974–986. doi:10.1159/000341474. ISSN 1421-9778. PMID 23221481.
  4. Fernandes, Hugo; Dechering, Koen; van Someren, Eugene; Steeghs, Ilse; Apotheker, Marion; Mentink, Anouk; van Blitterswijk, Clemens; de Boer, Jan (2010). "Effect of Chordin-Like 1 on MC3T3-E1 and Human Mesenchymal Stem Cells". Cells Tissues Organs. 191 (6): 443–452. doi:10.1159/000281825. ISSN 1422-6405. PMID 20130390. S2CID 39732221.
  5. Wang, Chuang; Xie, Xu-dong; Huang, Xun; Liang, Zhi-hong; Zhou, Chang-ren (August 2015). "A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan–collagen blend films at single cell level". Colloids and Surfaces B: Biointerfaces. 132: 1–9. doi:10.1016/j.colsurfb.2015.04.037. PMID 25996415.
  6. Deng, Jie; Zhao, Changsheng; Spatz, Joachim P.; Wei, Qiang (22 August 2017). "Nanopatterned Adhesive, Stretchable Hydrogel to Control Ligand Spacing and Regulate Cell Spreading and Migration". ACS Nano. 11 (8): 8282–8291. doi:10.1021/acsnano.7b03449. PMID 28696653.
  7. Andrée, Lea; Bertsch, Pascal; Wang, Rong; Becker, Malin; Leijten, Jeroen; Fischer, Peter; Yang, Fang; Leeuwenburgh, Sander C. G. (12 June 2023). "A Modular Platform for Cytocompatible Hydrogels with Tailored Mechanical Properties Based on Monolithic Matrices and Particulate Building Blocks". Biomacromolecules. 24 (6): 2755–2765. doi:10.1021/acs.biomac.3c00177. hdl:20.500.11850/618186. PMC 10265656. PMID 37222557.
  8. Johnston, E; Emani, C; Kochan, A; Ghebrehawariat, K; Tyburski, J; Johnston, M; Rabago, D (2020). "Prolotherapy agent P2G is associated with upregulation of fibroblast growth factor-2 genetic expression in vitro". Journal of Experimental Orthopaedics. 7 (1): 97. doi:10.1186/s40634-020-00312-z. PMC 7719583. PMID 33280075.

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