Manlius may refer to:
Places in the United States
- Manlius, Illinois
- Manlius (town), New York
- Manlius (village), New York
- Manlius Township (disambiguation)
Other uses
- Manlius (1826 ship)
- Manlius Pebble Hill, a school in DeWitt, New York
- any of various Romans belonging to the gens Manlia
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