Mary Walker may refer to:
- Mary Rebecca Dowd, birth name of Mamie Dowd Walker (1880–1960), American jurist and civil leader
- Mary Adelaide Walker, English travel writer
- Mary Ann Walker (1845–1888), Whitechapel murder victim
- Mary Broadfoot Walker (1888–1974), British physician
- Mary Chase Walker (1828–1899), American schoolteacher, pioneer, and suffragette
- Mary Cozens-Walker (1938–2020), English textile artist and painter
- Mary Edwards Walker (1832–1919), American physician and Medal of Honor recipient
- Mary Hardway Walker (1848–1969), Early civil rights activist and educator, resident of Chattanooga USA
- Mary L. Walker (born 1948), American lawyer
- Mary Lily Walker (1863–1913), Scottish social reformer
- Mary Richardson Walker (1811–1877), American missionary
- Mary Shore Walker (1882–1952), American mathematician
- Mary Walker (rodeo) (born 1959), American former world champion barrel racer
- Mary Willis Walker (1942–2023), American crime fiction author
Fictional characters
- Mary Walker, the alter-ego of the comic book character Typhoid Mary (comics)
- Mary Walker, in the US TV series Iron Fist, played by Alice Eve
See also
- Mary Walker Phillips (1923–2007), American artist, author, and educator
- Mary Walker-Sawka (born c. 1916), Canadian film producer
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