Middlesex is a historic county in southeast England.
Middlesex may also refer to:
Places
Australia
- Middlesex, Tasmania, a locality in Australia
- Middlesex, Western Australia, a locality of the Shire of Manjimup
Belize
- Middlesex, Belize, a village in Stann Creek District, Belize
Canada
Jamaica
United Kingdom
United States
- Middlesex, New Jersey
- Middlesex, New York
- Middlesex, North Carolina
- Middlesex, Vermont
- Middlesex, Virginia
- Middlesex Township, Butler County, Pennsylvania
- Middlesex Township, Cumberland County, Pennsylvania
- Middlesex School, Concord, Massachusetts
- Middlesex Canal, Massachusetts
- Middlesex County, Connecticut
- Middlesex County, Massachusetts
- Middlesex County, New Jersey
- Middlesex County, Virginia
- Middlesex Middle School, Connecticut
Literature
- Middlesex (novel), by Jeffrey Eugenides
Other
- Middlesex Plat Historic District, listed on the National Register of Historic Places in Polk County, Iowa
- Middlesex (1783 EIC ship), an East Indiaman of the British East India Company
- Middlesex County Cricket Club
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