Mitrohin, Mitrokhin (Russian: Митрохин; feminine Mitrohina, Mitrokhina) is a Russian patronymic surname derived from the first name "Mitroha", a diminutive form "Mitrofan". The surname may refer to:
- Dmitry Mitrohin (1883–1973), Russian artist
- Galina Mitrokhina (disambiguation)
- Vasili Mitrokhin (1922–2004), major and senior archivist for the Soviet Union's foreign intelligence service, author of the Mitrokhin Archive
- Sergey Mitrokhin (born 1963), Russian politician and statesman
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