Mitsotakis is a Greek surname. Notable people with the surname include:
- Mareva Grabowski-Mitsotakis (born 1967), Greek business executive
- Maria Mitsotáki (1907–1974), Athens socialite and a principal character in epic poem The Changing Light at Sandover
- Konstantinos Mitsotakis (1918–2017), Greek Prime Minister from 1990 to 1993
- Dora Bakoyannis (née Mitsotakis) (b. 1954), politician and Greek Foreign Minister; daughter of Prime Minister Konstantinos Mitsotakis
- Kyriakos Mitsotakis (b. 1968) Greek Prime Minister since 2019; son of Konstantinos Mitsotakis
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