N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase

N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (EC 3.5.1.103, MshB) is an enzyme with systematic name 1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol acetylhydrolase. This enzyme catalyses the following chemical reaction

1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H₂O

\rightleftharpoons

1-(2-amino-2-deoxy-alpha-D-glucopyranoside)-1D-myo-inositol + acetate

This enzyme mediates the rate limiting step in the mycothiol biosynthesis pathway.

References

Rawat M, Kovacevic S, Billman-Jacobe H, Av-Gay Y (May 2003). "Inactivation of mshB, a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis". Microbiology. 149 (Pt 5): 1341–9. doi:10.1099/mic.0.26084-0. PMID 12724395.
Newton GL, Av-Gay Y, Fahey RC (December 2000). "N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis". Journal of Bacteriology. 182 (24): 6958–63. doi:10.1128/jb.182.24.6958-6963.2000. PMC 94821. PMID 11092856.
Maynes JT, Garen C, Cherney MM, Newton G, Arad D, Av-Gay Y, Fahey RC, James MN (November 2003). "The crystal structure of 1-D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold". The Journal of Biological Chemistry. 278 (47): 47166–70. doi:10.1074/jbc.m308914200. PMID 12958317.

External links

N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside+deacetylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Hydrolases: carbon-nitrogen non-peptide (EC 3.5)
3.5.1: Linear amides / Amidohydrolases	Asparaginase Glutaminase Urease Biotinidase Aminoacylase ACY1 Aspartoacylase(ACY2) ACY3 Ceramidase Aspartylglucosaminidase Fatty acid amide hydrolase Histone deacetylase Sirtuin
3.5.2: Cyclic amides/ Amidohydrolases	Barbiturase Beta-lactamase Dihydroorotase
3.5.3: Linear amidines/ Ureohydrolases	Arginase Agmatinase Protein-arginine deiminase
3.5.4: Cyclic amidines/ Aminohydrolases	Guanine deaminase Adenosine deaminase AMP deaminase Inosine monophosphate synthase DCMP deaminase GTP cyclohydrolase I Cytidine deaminase AICDA Activation-induced cytidine deaminase
3.5.5: Nitriles/ Aminohydrolases	Nitrilase
3.5.99: Other	Riboflavinase Thiaminase II

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 3.5.1