Opening Night may refer to:
- Premiere, the debut (first public presentation) of a play, film, dance, or musical composition
Film
- The Opening Night, a 1927 American silent drama directed by Edward H. Griffith
- Opening Night (1977 film), an American drama by John Cassavetes
- Opening Night (2013 film), a film featuring Tuesday Knight
- Opening Night (2016 film), an American musical comedy directed by Isaac Rentz
Television
- Opening Night, a 1958 American television series consisting entirely of reruns of episodes of Ford Theatre
- Opening Night (TV series), a 1974–1975 Canadian television series
Episodes
- "Opening Night" (Curb Your Enthusiasm)
- "Opening Night" (Glee)
- "Opening Night" (Parenthood)
- "Opening Night" (Schitt's Creek)
- "Opening Night" (Smash)
Other uses
- Opening Night (album), a 2000 album by the Thad Jones/Mel Lewis Orchestra
- Opening Night (musical), a 2024 musical by Rufus Wainwright and Ivo van Hove
- Opening Night (novel), a 1951 novel by Ngaio Marsh
- Opening Night (video game), a 1995 edutainment computer game
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