Operation Buffalo may refer to:
- Operation Buffalo, a 1944 military operation, part of the Battle of Anzio in World War II
- Operation Buffalo (1956), four open-air nuclear tests at Maralinga, South Australia
- Operation Buffalo (1967), a Vietnam War operation
- Operation Buffalo (TV series), 2020 drama series based on the 1956 Operation Buffalo nuclear tests
See also
- Operation Büffel (Buffalo), a 1943 withdrawal of the German 9th Army
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